In the present study,we isolated an aquaporins gene designated MaPIP2-6 from banana.MaPIP2-6 ORF is 849 bp,which encodes 282 amino acids.Comparison of amino acid sequences and phylogenetic analysis indicated that MaPIP2-6 was closely related with OsPIP2-6.Subcellular localization assays showed that the MaPIP2-6 protein was present in the plasma membrane.Real-time quantitative polymerase chain reaction (QPCR) assay revealed that the expression of MaPIP2-6 was consistent after treatment of mannitol and NaCl,a slight decline in the early treatment,and then was induced and reach the maximum,then decreased,but was inverse after treatment of cold and ABA,and that MaPIP2-6 participated in the response of abiotic stresses at transcriptional level.These results indicated that MaPIP2-6 may be involved in abiotic stress response,which lays a foundation for further investigating the function of MaPIP2-6.