贵州山区3种木本植物无机碳利用特性的比较
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贵州省社会发展攻关项目(黔科合SY[2010]3043);国家自然科学基金(31301243,31070365,41201577);江苏大学引进人才科研启动基金(13JDG030)


Inorganic Carbon Utilization Traits of Three Woody Species Growing in the Mountain Area of Guizhou Province
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    摘要:

    以生长在喀斯特高原地区玉舍国家森林公园内的成熟银鹊树(Tapiscia sinensis)、白栎(Quercus fabri)和亮叶桦(Betula luminifera)为实验材料,通过对光合作用、叶绿素含量、叶绿素荧光参数、羧化效率(CE)、呼吸速率(Resp)、碳酸酐酶活性(WA)以及稳定碳同位素组成(δ13C)等指标的测定,分析3种植物不同的无机碳利用特性,为该区生态环境修复选择合适的建群植物种提供依据。结果显示:(1)银鹊树、白栎和亮叶桦分布都较为广泛,银鹊树生长的最佳土壤pH是4.5~5.5,而白栎更倾向于中性到弱酸性土壤,肥沃的酸性土壤则更有益亮叶桦生长;白栎和亮叶桦都能忍受干旱和贫瘠,但是银鹊树不能忍受干旱和高温。(2)银鹊树叶片的PnTrGs显著大于白栎和亮叶桦,亮叶桦和白栎的PnTrGs分别是银鹊树的69.5%、48.2%、66.7%和28.6%、21.7%、22.2%;亮叶桦叶绿素含量均为银鹊树和白栎的2倍,但3种植物间的WUE则无显著差异。(3)3个树种叶片净光合速率均随着CO2浓度升高呈持续上升的趋势,但它们之间的CO2补偿点和饱和点明显不同。其中,银鹊树和亮叶桦的CO2补偿点均低于50 μmol·mol-1,而白栎的则在250~300 μmol·mol-1之间;银鹊树的CO2饱和点在1 200 μmol·mol-1左右,亮叶桦则在2 300 μmol·mol-1左右,而白栎的CO2饱和点明显高于2 300 μmol·mol-1。(4)3个树种的CERespWA均为银鹊树>亮叶桦>白栎;而δ13C值则以银鹊树最低,亮叶桦和白栎较高。其中,白栎和亮叶桦的CERespWA分别为银鹊树的5.1%、25.7%、4.0%和45.3%、54.6%、6.8%,且树种间差异显著;白栎和亮叶桦的δ13C值显著高于银鹊树。研究表明,银鹊树能够吸收大气中的CO2或者在高活性碳酸酐酶作用下转化利用细胞内的HCO3-,它拥有较高的CO2利用能力及无机碳同化效率,因而能够拥有较高的产能;亮叶桦只能获取大气中的CO2作为无机碳源,但它对CO2的利用能力也较高,其产能仅次于银鹊树;白栎同样只能获取大气中的CO2作为无机碳源,同时它对大气中CO2的捕获、利用能力均低于银鹊树和亮叶桦,因而白栎生长非常缓慢,造成其本身对无机碳的需求也最低,所以其产能最低。

    Abstract:

    The mature Tapiscia sinensis,Quercus fabri and Betula luminifera which grew in the Yushe National Forest Park of karst plateau area were selected as experimental materials.Differences of photosynthetic inorganic carbon utilization traits between these three species were analyzed through the determination of photosynthesis,chlorophyll content,chlorophyll a fluorescence (Chlf),carboxylation efficiency (CE),respiration rate (Resp),carbonic anhydrase activity (WA) and stable carbon isotope ratios (δ13C),so as to select appropriate plants for community construction,finally achieve the recovery and restoration of this ecological environment.Results showed that:(1)T.sinensis,Q.fabri and B.luminifera were widely distributed.The optimal soil pH for T.sinensis was 4.5-5.5,while Q.fabri would prefer neutral to sub acidity soil and fertile acidic erinaceous soil was good for B.luminifera.Q.fabri and B.luminifera were resistant to drought and barren,but T.sinensis was not resistant to drought and high temperature.(2)Pn,Tr and Gs of leaf of T.sinensis were significantly higher than that of Q.fabri and B.luminifera.The Pn,Tr and Gs in B.luminifera and Q.fabri were only 69.5%,48.2%,66.7% and 28.6%,21.7%,22.2% of T.sinensis,respectively.The chlorophyll content of B.luminifera was twice of that in T.sinensis and Q.fabri,but WUE of them had no significant difference.(3)Net photosynthetic rates in leaves of these three plant species increased as CO2 concentration increased,but the CO2 compensation and saturation points between these three plant species were significantly different.CO2 compensation points of T.sinensis and B.luminifera were lower than 50 μmol·mol-1,while compensation point of Q.fabri was between 250-300 μmol·mol-1.CO2 saturation point of T.sinensis was approximately 1 200 μmol·mol-1,B.luminifera was approximately 2 300 μmol·mol-1,while saturation point of Q.fabri was significantly higher than 2 300 μmol·mol-1.(4)CE,Resp and CA activities of these three plant species were in the order:T.sinensis>B.luminifera>Q.fabri;while δ13C of T.sinensis was the lowest,while the values of δ13C of B.luminifera and Q.fabri were higher.CE,Resp and CA activities in Q.fabri and B.luminifera were only 5.1%,25.7%,4.0% and 45.3%,54.6%,6.8% of T.sinensis,respectively,and they varied significantly with different plant species.The values of δ13C of Q.fabri and B.luminifera were significantly higher than that of T.sinensis.The results indicated that T.sinensis could obtain inorganic carbon from atmospheric CO2 or intracellular bicarbonate by CA with higher activity and its CO2 utilization ability and inorganic carbon assimilation efficiency was also higher,as a result,its production was higher.B.luminifera could only absorb inorganic carbon from atmospheric CO2,but it had higher CO2 utilization ability,its production was lower than that of T.sinensis but higher than that of Q.fabri.Q.fabri could only absorb inorganic carbon from atmospheric CO2 too,its CO2 capture and utilization ability was also lower than that of T.sinensis and B.luminifera.Its requirement of inorganic carbon was the least.So it grew slowly,and its production was the lowest.

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邢德科,吴沿友,王 瑞,等.贵州山区3种木本植物无机碳利用特性的比较[J].西北植物学报,2015,35(3):579-586

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  • 在线发布日期: 2015-04-13
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