In this study,the wild banana in Fuzhou with stronger anti-cold ability was used as materials to clone the iron superoxide dismutase family (FeSOD) genes.Two FeSOD genes with four transcripts,designed as MuFSD1A(Accession No.JX844026),MuFSD1B(Accession No.KJ786318),MuFSD1B-variant1(Accession No.KJ786319) and MuFSD1B-variant2(Accession No.KJ786320),respectively,were obtained by RACE and RT-PCR.The full-length cDNA sequences of MuFSD1A were 1 277 bp,encoding a 300 aa polypeptide,while that of the MuFSD1B were 1 378 bp,encoding 260 amino acids.Genomic structure analysis revealed that MuFSD1B-variant1 and MuFSD1B-variant2 were alternatively spliced variants of MuFSD1B.Bioinformatics and subcelluar localization analyses indicated that both MuFSD1A and MuFSD1B were mainly located in chloroplast,but their physicochemical properties,phosphorylation sites and protein structures were different from each other.Sequence alignments showed that MuFSD1A and MuFSD1B shared a low sequence identity (33.33%),although they all carried the conserved metal ligand binding sites and residues essential for distinguishing FeSOD from MnSOD.Phylogenetic analysis suggested MuFSD1A and MuFSD1B are grouped into different clusters.qRT-PCR results revealed that cold stress induced the expression of MuFSD1A but suppressed MuFSD1B expression.In addition,the expression level of MuFSD1A was significantly enhanced with the temperature decreasing,indicating this gene member might play a major role in banana cold resistance.