The anatomical structure and relationship to accumulation with alkaloid in vegetable organs of Dictamnus dasycarpus were studied,with plant anatomy,histochemistry and phytochemistry.The results show that:(1)the structures of perennial root,stem and leaf are in agreement with anatomy of the common dicotyledons.The perennial root consists of periderm,secondary phloem,vascular cambium and secondary xylem,and plenty of starch grains,calcium oxalate cluster crystals,fibers and oil cells are also observed.The stem constitutes epidermis,cortex,vascular tissue and pith.The leaf consists of epidermis,palisade tissue,spongy tissue and vein.The phloem fibers are detected at the location of primary phloem.The capitate glandular hairs and non-glandular hairs are located on the epidermis of stem and leaf,and the secretory cavities distribute in the mesophyll tissue which are closed to the epidermis.(2)Histochemically,the alkaloids are located in the periderm,parenchyma cells of secondary phloem,vascular cambium and parenchyma cells in xylem of perennial root.In stem,the alkaloids are detected in the epidermis,cortex,phloem,parenchyma cells of xylem,parenchyma cells surrounding the pith in stem.In leaf,the alkaloids are in the epidermis,parenchyma cells of the vein and phloem.In addition,the alkaloids are also detected in the secretory cavities and glandular hairs.(3)The HLPC results suggest that the content of dictamnine in Dictamni Cortex,and the xylem,stem and leaf are 0.041%,0.012%,0.004% and 0.002%,and the xylem also shows high content of dictamnine as some other regions.The study suggests that the xylem,stem and leaf are also characterized by certain content of dictamnine,indicating a potential exploitation and utilization value.