5种速生柳无性系对Pb2+胁迫的生理抗性比较
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天津市农业科技成果转化项目(201102060)


Physiological Resistance of Five Fast-Growing Willow Clones to Lead (Pb) Stress
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    摘要:

    以5种一年生速生柳(竹柳、苏柳172、苏柳795、旱快柳、旱柳)幼苗为材料,通过盆栽铅胁迫实验,测定其生长过程中的生理生化指标,分析其对铅胁迫的生理响应及耐受性。结果表明:(1)随着Pb2+浓度的增加,5种速生柳叶片保护酶SOD、POD和CAT活性均呈先升高后降低的趋势,其中竹柳的3种保护酶活性均为最高。(2)随着Pb2+浓度的增加,各无性系叶片中渗透性调节物质可溶性蛋白、脯氨酸和可溶性糖含量均呈先上升后下降的趋势,竹柳和苏柳172在高Pb2+浓度(1500 mg·kg-1)胁迫下表现出对Pb2+的强耐受性。(3)随着Pb2+浓度的增加,各速生柳无性系叶片丙二醛含量均呈逐渐增高的趋势,但增幅有所不同,竹柳增幅最小,旱柳增幅最大。(4)随着Pb2+浓度的增加,5种速生柳叶片中叶绿素含量均呈先上升后下降的趋势,但各无性系的下降幅度存在差异,其中竹柳的下降幅度最小,旱柳下降幅度最大。(5)随着Pb2+浓度的增加,5种速生柳根系活力都呈先上升后下降的趋势,其中竹柳的根系活力最强,苏柳795的最弱。(6)各速生柳根系对铅均有一定的吸附能力,竹柳对铅积累量最大。(7)对各生理指标的Fuzzy数学隶属函数综合评价结果表明,5种速生柳对Pb2+耐受性强弱表现为:竹柳>旱快柳>旱柳>苏柳795>苏柳172。

    Abstract:

    The effects of lead (Pb) stress on some physiological indicators of five fast-growing willow clones (Salix ‘Zhuliu’,Salix jiangsuensis CL J-172,Salix jiangsuensis CL J-795,Salix matsudana var.anshanenensis and Salix matsudana Koidz) were studied in a pot experiment.The results showed that:(1)the POD,SOD and CAT activities of five clones increased initially and then decreased with the increase of lead concentrations;The Salix ‘Zhuliu’ was better than other four clones;(2)with the increases of lead concentrations,soluble protein,proline and soluble sugar contents of five clones significantly increased at the lower lead concentrations,and reduced at the higher concentrations.Salix ‘Zhuliu’ and Salix jiangsuensis CL J-172 had higher resistance to lead stress compared to other three clones at 1 500 mg·kg-1 of lead;(3)MDA content of five clones generally increased with the increase of lead concentrations,but the increase rate was different (The lowest and the highest were Salix ‘Zhuliu’ and S.matsudana Koidz);(4)with the increase of lead concentrations,chlorophyll content of five clones increased initially and then decreased;Salix ‘Zhuliu’ has seen its decrease rate less than those of other four clones at 1 500 mg·kg-1 of lead;(5)the root activity of five clones increased initially and then decreased with the increase of lead concentrations;The root activity of Salix ‘Zhuliu’ clone was the strongest;(6)the accumulation to Pb2+ of five willows had different performance under the same lead concentration,and the Salix ‘Zhuliu’ was better than others;(7)comprehensive evaluation of each physiological parameters indicated that the lead ions tolerance order are:indicated that:Salix ‘Zhuliu’>Salix matsudana var.anshanenensis>Salix matsudana Koid>Salix jiangsuensis CL J-795>Salix jiangsuensis CL J-172.

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杨 斌,张文辉,高建欣.5种速生柳无性系对Pb2+胁迫的生理抗性比较[J].西北植物学报,2015,35(6):1182-1189

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  • 在线发布日期: 2015-08-09
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