In order to clone VdWRKY53 transcription factor from ‘Vitis davidii 0943’,reveal the relationships between its sequence signature,gene function and the resistance to white rot fungi and reveal the molecular regulatory mechanisms of resistant germplasm preliminary,we designed primers and cloned VdWRY53 of ‘Vitis davidii 0943’ based on the known homologous sequences VvWRKY53.Its expression was verified through bioinformatic analysis of genes,promoters and real-time fluorescence quantitative PCR after inoculating white rot fungi and spraying salicylic acid.The expression of WRKY53 can be monitored in both ‘Vitis davidii 0943’ and ‘Pinot Noir’ grape which inoculate white rot fungi or salicylic acid by real-time fluorescence quantitative PCR verification.The CDS and promoter of the Chinese wild grape ‘Vitis davidii 0943’ VdWRKY53 both have the resistance genes feature.At the same time VdWRKY53 is different from the ‘Pinot Noir’ grape and the nucleic and animo acids are also different.These differences may cause different functions.VdWRKY53 plays an important biological function in grape disease resistance by bioinformatic analysis and real-time fluorescence quantitative PCR.