To obtain tissue-cultured plant,We used Tamarix chinensis seeds as materials and established their sterile solated culture system by tissue culture method.A stress condition was formed through adding different concentrations of NaCl (0,50,80,100,120,150 and 200 mmol/L) to the medium (MS+1.0 mg/L 6-BA+0.1 mg/L NAA).To explore the physiological mechanism of T.chinensis tissue-cultured plant response to NaCl-stress,We investigated the changes of activities of T.chinensis tissue-cultured plant antioxidases,contents of osmotica,plasma membrane permeability and production rate of super oxygen anion (O^-·₂) under different concentrations of NaCl-stress.The results indicated that:(1)The activities of superoxide dismutase (SOD) and peroxidase (POD) of T.chinensis tissue-cultured plant were rising and then going down as concentrations of NaCl-stress were increasing,and both at 100mmol/L NaCl-stress reached a maximum,whereas,the activity of ascorbic acid peroxidase (APX) was gradually declining.(2)The contents of osmotica such as free proline (Pro),soluble protein (Pr) and soluble sugar of tissue-cultured plant were gradually rising and significantly higher than that of the concurrent control as concentrations of NaCl-stress were increasing,and at 200 mmol/L concentration notably increased 53.77%,47.38% and 67.65%,respectively.(3)The production rates of hydrogen peroxide (H₂O₂) and super oxygen anion (O^-·₂),content of malonic dialdehyde (MDA) and plasma membrane permeability of tissue-cultured plant were all gradually rising and significantly higher than that of the concurrent control as concentrations of NaCl-stress were increasing,and at 200 mmol/L concentration notably increased 36.42%,58.71%,82.60% and 42.54%,respectively.The study confirmed that antioxidases system of T.chinensis tissue-cultured plant showed favorable active oxygen scavenging activity under a low concentration of NaCl-stress (≤100 mmol/L) and the osmotica played positively regulated functions under different concentrations of NaCl-stress.Accordingly,T.chinensis showed a strong salt tolerance.