外源亚精胺对盐胁迫下菠菜叶绿素合成前体含量的影响
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现代农业产业技术体系建设专项资金(CARS25C03);江苏省农业“三新”工程项目(SXGC[2015]321;SXGC[2015]270);南京农业大学植物生产国家级实验教学中心开放性实验项目(ZKF201302);江苏省重大创新载体建设项目(BY2011012);江苏省前瞻性联合研究项目(BY2013071)


Effects of Exogenous Spermidine on Chlorophyll Precursors Content of Spinach Plants under Salt Stress
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    摘要:

    采用营养液水培的方法,以耐盐性较弱的菠菜(Spinacia oleracea L.)品种‘全能菠菜’为试材,研究外源亚精胺(Spd)对盐胁迫下菠菜生长及叶绿素前体含量的影响。结果显示:(1)菠菜植株在250 mmol·L-1NaCl胁迫下生长受到显著抑制,单株总鲜重、总干重、地上部鲜重、地上部干重、地下部鲜重和地下部干重分别较对照降低57.23%、53.08%、63.14%、55.05%、42.22%和42.86%,叶面喷施1.0 mmol·L-1Spd使其分别比盐胁迫处理提高62.83%、71.19%、60.57%、71.74%、70.31%和69.23%;(2)250 mmol·L-1 盐胁迫使菠菜叶片叶绿素a(Chl a)、叶绿素b(Chl b)及总叶绿素Chl(a+b)含量分别较对照显著降低42.31%、54.55%和44.53%,叶面喷施1.0 mmol·L-1 Spd使其分别较单纯盐胁迫处理显著提高46.24%、51.85%和47.11%;(3)盐胁迫使菠菜叶片Chl a、Chl b、叶绿素酸(Pchl)、镁原卟啉Ⅸ(Mgproto Ⅸ)、原卟啉Ⅸ(Proto Ⅸ)和尿卟啉 Ⅲ(Uro Ⅲ)含量均显著降低,而使胆色素原(PBG)和δ氨基酮戊酸(ALA)含量升高,而叶片Chl a、Chl b、Pchl、Mgproto Ⅸ、Proto Ⅸ和Uro Ⅲ含量在施用外源Spd后均显著提高,而其PBG和ALA含量有所降低。可见,盐胁迫条件下,菠菜叶片叶绿素合成受阻,受阻位点在PBG向Uro Ⅲ的转化过程,外源Spd能够缓解盐胁迫下菠菜叶片叶绿素合成受阻的程度,促进叶绿素合成,从而提高叶绿素含量。

    Abstract:

    Effect of exogenous spermidine(Spd)on growth and chlorophyll precursors content of spinach plants under NaCl stress was studied by hydroponics using ‘cv.Quanneng’ of spinach,a sensitive cultivar to salt.The results showed that:(1)growth of spinach plants was inhibited significantly by 250 mmol·L-1 NaCl,as a result of total fresh weight,total dry weigh,shoot fresh weight,shoot dry weigh,root fresh weight and root dry weigh of spinach plants were significantly decreased by 57.23%,53.08%,63.14%,55.05%,42.22% and 42.86%,respectively,as compared with control.However,plant growth was enhanced by 1.0 mmol·L-1 Spd,those indexes increased by 62.83%,71.19%,60.57%,71.74%,70.31% and 69.23%,respectively,as compared with salt stress treatment.(2)Chlorophyll a(Chl a),chlorophyll b(Chl b)and total chlorophyll[Chl(a+b)] contents in leaves of spinach plants were decreased by 250 mmol·L-1 salt stress to 42.31%,54.55% and 44.53%,respectively,but those were increased by 1.0 mmol·L-1 Spd to 46.24%,51.85% and 47.11%,as compared with salt stress treatment.(3)Chlorophyll precursors such as Chl a,Chl b,protochlorophyll(Pchl),Mgprotoporphyrin Ⅸ(Mgproto Ⅸ),protoporphyrin Ⅸ(Proto Ⅸ)and uroorphyrinogen Ⅲ(Uro Ⅲ)were decreased by salt stress,but were increased by Spd.However,others such as δaminolevulinic acid(ALA)and porphobilinogen(PBG)were enhanced by salt stress,but were suppressed by Spd.These results suggested that chlorophyll synthesis process from PBG to Uro Ⅲ was inhibited by salt stress,and this inhibition was alleviated by exogenous Spd,as a result of chlorophyll increasing.

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王 颖,郭世荣,束 胜,等.外源亚精胺对盐胁迫下菠菜叶绿素合成前体含量的影响[J].西北植物学报,2015,35(10):2026-2034

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  • 在线发布日期: 2015-11-02
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