6种丁香花挥发性成分的动态顶空吸附ATD-GC/MS分析
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北京市属高等学校创新团队建设与教师职业发展计划(IDHT20150503);国家自然科学基金(31071817);北京市教委科技提升计划(PM2014014207)


Analysis of Floral Volatile Components from 6 Syringa Plants Collected by Dynamic Headspace Sampling by ATD-GC/MS
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    摘要:

    丁香是一种重要的芳香木本观赏植物,花开繁茂,芳香浓郁。该研究采用活体植物动态顶空套袋-吸附法收集了香雪丁香、花叶丁香、关东丁香、蓝丁香、小叶丁香、四季蓝丁香6种不同丁香花的挥发物,通过自动热脱附-气质联用分析技术分析丁香花的挥发物成分,为研究其花香释放机理、进一步开发利用丁香资源及新品种选育提供依据。结果表明:(1)在6种丁香花挥发物中共检测到80种成分,分别属于苯形烃、萜类、醇、醛、酮、脂肪烃、酯、酸及其它类。(2)花叶丁香花的挥发物释放量最高,且为释放量最低的四季蓝丁香的1.8倍。(3)除香雪丁香外其他5种丁香花的挥发物中苯形烃在9类挥发物成分中含量最多,而萜类是香雪丁香花挥发物的主要成分。(4)乙苯、间二甲苯、对二甲苯、二丁基羟基甲苯、2-乙基-1-己醇、5-乙基-2,2,3-三甲基庚烷这6种成分在不同丁香花挥发物成分中含量均较高,是6种丁香花挥发物的主要成分。

    Abstract:

    Syringa is an important woody ornamental plant with lush flowers and aromatic scent.The research on volatile compositions is necessary for floral scent release mechanism,and provides scientific basis for botanical garden application and new varieties breeding.In this study,the floral volatiles emitted from 6 different Syringa species and varieties including S.oblata×S.vulgaris ‘Xiangxue’,S.persica,S.velutina,S.meyeri,S.pubescens subsp.microphylla and S.meyeri ‘Si Ji Lan’ were collected with dynamic headspace method,and then were identified by automated thermal desorption-gas chromatography / mass spectrometry (ATD-GC/MS) technique.The results showed that:(1) the floral volatile components of the 6 different Syringa species and varieties were distinct qualitatively and quantitatively.A total of 80 volatile components were identified,and these compounds belonged to 9 volatile categories including benzenoid and derivative,terpenoid,alcohol,aldehyde,ketone,fatty hydrocarbon,ester,acid,and others.(2) Among the 6 different species,the highest release amount of volatile components was emitted from S.persica,which was almost 1.8 times more than the lowest that from S.meyeri ‘Si Ji Lan’.(3) Except S.oblata×S.vulgaris ‘Xiangxue’,benzenoid and derivative showed the highest release amount among the 9 categories,so benzenoid and derivative was the main volatile category of the floral fragrances.In S.oblata×S.vulgaris ‘Xiangxue’,the release amount of terpenoid was the highest among 9 categories.(4) Among the components,1,3-dimethyl benzene,2-ethyl-1-hexanol,ethylbenzene,p-xylene,butylated hydroxyl toluene,5-ethyl-2,2,3-trimethyl heptane were exhibited higher release amount in these Syringa plants,which suggested that these compounds were main volatile components of lilac flowers.

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秦 颖,杨晓霞,冷平生,等.6种丁香花挥发性成分的动态顶空吸附ATD-GC/MS分析[J].西北植物学报,2015,35(10):2078-2088

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  • 在线发布日期: 2015-11-02
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