Spacer length (the distance between connected ramets) may cause significant effects of physiological integration,but lack of correlated studies.The change of antioxidant system and photosynthetic pigment may affect plant response to the moisture heterogeneity,but lack of related research also.The experiment with connected Indocalamus decorus clonal ramets under 3 substrate relative water content [high,(90±5)%;intermediate (60±5)%;low (30±5)%] and 2 water potential gradient (H-L and M-L),the antioxidant enzyme,content of soluble protein,MDA and photosynthetic pigment of three spacer length (10,25 and 40 cm) connected clonal ramets were determined.The results showed that:(1)CAT activity,leaf relative electric conductivity and MDA content increased at first and then decreased,while SOD activity presented a trend just on the contrary to the CAT activity with the increasing of treatment time.Overall,POD activity and photosynthetic pigment content present “N” type,while soluble protein content showed inverted “N” trend with treatment time extended.(2)Water potential gradients and spacer length affected the water physiological integration of Indocalamus decorus clonal ramets.Antioxidant enzyme activities,soluble protein and photosynthetic pigment contents of low water potential receptor ramets decreased,while leaf relative electric conductivity and MDA content increased significantly with the water potential difference increasing and spacer distance shortening between I.decorus ramets.The water potential is higher and the spacer is longer,the antioxidant enzyme activities,soluble protein and photosynthetic pigment contents of donor ramets are higher and the leaf relative electric conductivity and MDA content are lower.All results indicated that water transfer from high water potential ramets to low water potential ramets would be taken place in I.decorus clonal system under heterogeneous water environment.The water potential difference between ramets is bigger,and the length of spacer is shorter,the intensity of physiological integration is stronger,the benefit of receptor ramets is more obvious.Moreover,with treatment time extending,water integration intensity between connected clonal ramets increased in early time,but it weakened later,which reflected that the consumption-benefit effect of donor ramets and receptor ramets changed in time dimension,and the change was much more obvious in early stage of the treatment.The research provided a theoretical reference for artificial water management of commercial bamboo forests.