Flue-cured tobacco ‘Nanjiang 3’ was a kind of main cultivated varieties in Guizhou Province.Paraffin section method was employed to observe its anatomical structure of microsporogenesis,megasporogenesis and development of female and male gametophytes.The results showed that,the anther wall is defined as basic development type with glandular veriform tapetum.The cytoplasm of microspore mother cell undergoes simultaneous division and produces tetrahedral or decussate tetrad.The mature pollen belongs to 2 cell type and has three or four apertures.The ovary of flue-cured tobacco ‘Nanjiang 3’ is anatropous with thin nucellus.The archesporial cell develops to megaspore mother cell directly and has larger volume.Four megaspore cells form a linear tetrad.The development pattern of embryo sac is polygonum type,finally forms 7-cell or 8-cell nucleate mature embryo sac.Stamen develops earlier than gynoecium and the period of microsporogenesis,megasporogenesis and development of female and male gametophytes can be judged by appearance from the flower bud.