UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响
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国家自然科学基金项目(31160139)


Influence of Enhanced UV-B on Physiological Characteristics of Eupatorium adenophorum L.and Artemisia argyi Levl
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    摘要:

    以外来入侵杂草紫茎泽兰(Eupatorium adenophorum L.)和本地土著杂草艾草(Artemisia argyi Levl)为材料,以自然光照为对照,用增强UV-B(0.46 mW·cm-2·h-1)对材料进行7周辐射处理,随后停止辐射增强进行恢复试验,观察2种植物的生长状况,测定分析其光合特性、氧化物和抗氧化物含量、抗氧化酶活性的变化,探讨在紫外UV-B辐射增强条件下2种植物光合作用、光保护及损伤修复能力的差异。结果表明:(1)随着辐射时间的增长,2类植物叶片开放的PSⅡ反应中心激发能捕获效率(Fv′/Fm′)和PSⅡ凌晨最大潜在最大光化学效率(Fv/Fm)受到轻微抑制,但紫茎泽兰最大净光合速率(Pn max)受到的抑制比艾草更明显。(2)2类植物叶片内过氧化产物丙二醛(MDA)和超氧基离子(O2)的含量增加,但艾草中O2的相对累积比例比紫茎泽兰要高很多。(3)紫茎泽兰的抗氧化物抗坏血酸(ASA)和谷胱甘肽(GSH)的含量先增加后减少,艾草中的含量则保持在更高水平;2种植物的过氧化物酶(POD)和超氧化物歧化酶(SOD)活性都显著增强,抗坏血酸过氧化物酶(APX)活性都先增强后减弱,紫茎泽兰的过氧化氢酶(CAT)活性逐渐被抑制,而艾草则是先增强后减弱。研究认为,紫茎泽兰和艾草对UV-B辐射增强的光合作用和抗氧化系统响应过程类似但存在差异,对增强UV-B辐射环境的适应能力及抗逆性均较强。

    Abstract:

    To investigate the differences in photosynthesis,protective ability and repair capability between the invasive weed Eupatorium adenophorum L.and the native weed Artemisia argyi Levl under the condition of enhanced UV-B radiation,we designed a seven-week experiment under the condition of UV-B radiation up to 0.46 mW·cm-2·h-1 and an extra experiment after stopping radiation to observe the changes in both species’ growth conditions.Moreover,the effects on photosynthetic capacity,the contents of oxides and antioxidants as well as the activity of antioxidant enzymes were measured and analyzed.And the results showed as follows:(1)the maximum photosynthetic assimilation rate (Pn max),photochemical efficiency in the light and maximal quantum yield of PSⅡ were restrained,but the former was showed more repressive significantly in E.adenophorum L.as the durations of UV-B radiation increased.(2)The contents of malonaldehyde (MDA) and super-oxygen ion (O2) in both species were accumulated as the durations of UV-B radiation increased,but the increased proportion of them was more significant in A.argyi Levl.(3)The contents of antioxidants concluding ascorbate (ASA) and glutathione (GSH) turned out different tendencies that the contents tended to firstly increased and then decreased in E.adenophorum L.while to increased and maintained a high level in A.argyi Levl.Besides,the enzymatic activities of peroxidase (POD) and superoxide dismutase (SOD) were improved remarkably after radiation in both species.And the enzymatic activity of ascorbate peroxidase (APX) in both species firstly increased and then decreased.As for the enzymatic activity of catalase (CAT),it was gradually inhibited in E.adenophorum L.but firstly enhanced and then inhibited in A.argyi Levl.As a consequence,we found the physiological mechanism of photosynthesis and antioxidation of E.adenophorum L.and A.argyi Levl responded to enhanced UV-B radiation were similar with some differences.

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李良博,张连根,唐天向,等. UV-B辐射增强对紫茎泽兰和艾草抗性生理特性的影响[J].西北植物学报,2016,36(2):343-352

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  • 在线发布日期: 2016-03-29
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