Sequencerelated amplified polymorphism technique was adopted to analyze 21 pomegranate accessions. Sixteen primer combinations with clear band patterns and polymorphism were selected from thirtysix primer combinations. 271 loci were detected by sixteen pairs of SRAP primers. Among them 194 were polymorphic, the polymorphic rate was 71.59％, an average of 16.94 loci and 12.13 polymorphic loci were amplified by each primer pair. The genetic similarity coefficient of 21 pomegranate accessions ranged from 0.63 to 0.89, with an average of 0.77. An unweighted pair group method with arithmetic mean (UPGMA) tree established from Jaccard similarity coefficients suggested that 21 pomegranate accessions were clustered into five subgroups. The average value of observed number of alleles, effective number of alleles, Neis gene diversity index, Shannons information index are 1.712 2, 1.352 8, 0.199 9 and 0.310 5, respectively. There was rich genetic diversity among the pomegranate accessions in Kashi Prefecture. The clustering results using SRAP markers were consistent with the phenotypic characteristics of pomegranate. Sequencerelated amplified polymorphism (SRAP) is a simple and effective method to research on genetic diversity of pomegranate.