Plant prolinerich proteins (PRPs) are putative cell wall proteins, which are usually associated with different abiotic stresses. The expression of SbPRP3 under abiotic stress treatments in soybean was detected by realtime fluorescence quantitative PCR. The expression of SbPRP3 increased under salt treatment, while it increased firstly and thereafter declined under drought and cold treatments. Furthermore，the ORF of SbPRP3 was cloned into the plant expression vector of pRI101AN and then introduced into tobacco by Agrobacteriummediated transformation. Three positive transgenic tobacco plants were obtained. The transgenic tobacco plants were treated with salt, drought and cold stresses. The results showed that the transgenic plants maintained higher levels of proline and lower level of malondialdehyde compared to wildtype plants under salt and cold stresses. While the levels of proline and malondialdehyde in transgenic plants had no significant differences compared to wildtype plants under drought stress. These data indicated that the transgenic tobacco plants constitutively expressing SbPRP3 showed an increased tolerance to salt and cold compared to wildtype plants.