The two samples of truffles collected in the Helan Mountain region of Inner Mongolia were studied by Anatomy and molecular biology. (1) Two strains of ascoma were tan, smooth surface, no obvious verrucous protuberance and edges. (2) Gleba of strain a is ivory, dense and vagus veins. Hyphae organization is brown; Spherical or rodshaped ascus in honeycomb arrangement contained 1-4 ascospore with package; the surface ascospore had a protuberant ornamentation and was spherical. Thickness of double wall was about 1.7 um, diameter 20 microns (including ascospore grain). (3) Gleba of strain b had cracks, loose, in addition to the above ascus and spore, also possessed a special ascus like “pocket” containing a large number of sharp, smooth, brown ellipsoidal spores. (4) Molecular evolution analysis shows that the two strains into one, but there is a relatively low approval ratings for genus Tuber. Therefore, the two strains were concluded that they might be a new record species of genus Choiromyces in China.