The experiment was carried out under artificial greenhouse condition to investigate the effects of growth and pseudobulb’s organic matter content of Pleione bulbocodioides with three temperature treatments（20 ℃/15 ℃，15 ℃/10 ℃ and 10 ℃/5 ℃）. The results showed that: (1) compared with 20 ℃/15 ℃ treatment (simulate the natural temperature of the native place during the flowering period), the early flowering time with 15 ℃/10 ℃ and 10 ℃/5 ℃ treatments were advanced 24 days and 53 days respectively, while the flowering was prolonged 4 days and 6 days respectively. (2) With 10 ℃/5 ℃ treatment，the color of flower was deeper than that of other treatments,while many dumb flower buds were exist. (3) With the process of the old pseudobulb growth, the starch content showed a trend of increase under 20 ℃/15 ℃ treatment，and fall after rise under 15 ℃/10 ℃ and 10 ℃/5 ℃ treatments; Under the three temperature treatments, the maximum of soluble sugar content all appeared in flowering period; Both the soluble sugar and soluble protein were maintained high level under 10 ℃/5 ℃ treatment. Studies had shown that both 20 ℃/15 ℃ and 15 ℃/10 ℃ treatments were favorable to the growth and flowering of P. bulbocodioides, while 10 ℃/5 ℃ treatment not. The dormant pseudobulb of P. bulbocodioides doesnt need low temperature induction break dormancy and would start the corresponding growth process when temperature rises.