In this study, the bZIP gene fragment from a digital expression profiling of cotton root tissue infected by Fusarium oxysporum f. sp. vasinfectum (Fov) was cloned from the roots of Zhongmiansuo 12 through the in silico cloning and RTPCR, named as GhTGA2.2 (Genebank: KY069278). Sequence analysis showed that the cDNA of GhTGA2.2 gene was 1 356 bp, which encoded a protein of 452 amino acids containing a conserved bZIP domain with predicted molecular weight of 50.04 kD and an isoelectric point of 5.85. Phylogenetic analysis revealed that GhTGA2.2 belonged to the TGA transcription factors of the bZIP subfamily and was related closely to AtTGA2 of Arabidopsis thaliana and NtTGA2.2 of Nicotiana tabacum. qRTPCR analysis indicated that, the relative expression of GhTGA2.2 increased in the resistant cultivar after the treatment of Fov. The expression level increased first and decreased then with the treatment time going on, and the expression level reached the highest at over 24 hours after treatment; After the treatment of salicylic acid, the level of GhTGA2.2 expression reached rapidly the maximum at 1 h. While the relative expression of GhTGA2.2 decreased after the ethylene and jasmonic acid treatments; we speculated that GhTGA2.2 may involve in salicylic acid signal pathway to defense Fov.