A fulllength cDNA sequence of SEPALAATA(SEP) gene was obtained from inflorescence tissue of mango (Mangifera indica cv. Carabao) with homologybased cloning and RACE, named MSEP1 (GenBank accession no.KP702299). The full length of MSEP1 is 921 bp containing 726 bps open reading frame, encoding 241 amino acids with molecular weight 27.7 kD and isoelectric point 5.79. Sequence alignment and phylogenetic tree analysis indicated that MSEP1 deduced protein contained a conservative MADSbox and semiconservative K domain and belonged to the SEP subfamily of the MADSbox family. Tissuespecific analysis showed that MSEP1 was high expression in leaves and flowers especially in inflorescences, low expression in roots and stems of mango. MSEP1 may have important roles in reproductive growth of mango.