In this paper, two NAC genes were cloned from the shoots of Bambusa emeiensis, based on the transcriptome database of B. emeiensis. They were named BeNAC3 and BeNAC4. Their registration number were KU821587 and KU821588 in GenBank, respectively. The tissue expression and transcriptional activity of BeNAC3 and BeNAC4 were studied on the basis of bioinformatics analysis. The results shown that both genes have an obvious transmembrane domain, which could be a kind of membrane binding protein, based on the analysis of TMHMM and phylogenetic. Also, they are highly homologous to the NAC2 subfamily and the ANAC011 subfamily, respectively. Moreover, there is a speculation that BeNAC3 might be related to the regulation of hormone synthesis and flowering according to its relative expression in bamboo shoots, unexpanded leaves, expanded leaves and stems, and its relationship with the NAC2 subfamily in evolutionary trees. As for BeNAC4, it might be involved in the secondary metabolism, for its expression in mature tissues was significantly higher than that in young shoots and unexpanded leaves. The experiments of single hybrid transcription activity in yeast indicated that both BeNAC3 and BeNAC4 could activate the expression of the βGAL gene (lacZ), which indicated that both of them have a particular transcriptional activity. The research results lay a foundation for the future functional verification of transgenic plants from B. emeiensis.