Combining a DH（Double Haploid）lines（348 lines）in Brassica napus and constructed genetic linkage group，we detected and analyzed QTL for thousandseed weight (TSW) by composite interval mapping model in 2009-2013 in Dali of Shaanxi Province. A total of 46 significant identified QTLs for TSW were obtained and mainly located on A7, C1 and C6 chromosomes, etc. The maximum of phenotypic variation was qTSW09DL111 which reached to 19.63%, and the minimum of phenotypic variation was qTSW11DL9 which only explained 2.73%. The identified QTLs were integrated into consensus QTLs by metaanalysis and cqTSWC12 reached to 10.64% of phenotypic variation. Some consensus QTLs were repeatedly detected, cqTSWC13, for example, was repeatedly detected in five consecutive years, indicating QTLs for TSW were expressed stably in different environments. Meanwhile, the major QTL cqTSWC12 was regarded as a new QTL on C1 chromosome. The genomic information of these QTLs will be conducive for the construction of near isogenic line, fining mapping and Mapbased cloning. The results provide an important theoretical guidance for molecular breeding and developing high seed yield varieties in Brassica napus.