辣椒ARF基因家族的鉴定与表达分析
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国家自然科学基金(31470105);


Identification and Expression Analysis of ARF Gene Family in Pepper
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    摘要:

    为进一步研究辣椒ARF(Auxin Response Factor)基因家族在其生长发育及逆境胁迫中的作用,利用生物信息学方法从辣椒基因组中鉴定出20个ARF基因,这些基因不均匀地分布在辣椒12条染色体上。进化关系显示辣椒ARF基因可分为ClassⅠ和Ⅱ两大类,进一步可细分为Ⅰa、Ⅰb、Ⅰc、Ⅰd、Ⅰe、Ⅱa和Ⅱb等7个亚类。基因结构图表明,该基因家族由1~15个外显子构成,且各基因在不同发育时期不同组织中的表达量不同,具有一定的特异性。qRTPCR结果表明,高盐胁迫可以明显激活或抑制ARF基因家族的表达。

    Abstract:

    20 ARF genes were identified from the capsicum genome by bioinformatics, and these genes were not welldistributed on 12 chromosomes of pepper. The results of the phylogeny relationship showed that ARF genes of pepper could be divided into ClassⅠand Ⅱ, and could be further divided into seven subgroups: Ⅰa, Ⅰb, Ⅰc, Ⅰd, Ⅰe, Ⅱa andⅡb. The results of gene structure showed that ARF gene family members were composed of 1~15 exons, and for each gene expression level of different tissues in different developmental stages revealed that the gene family had certain expression specificity. The qRTPCR uncovered that high salt stress could significantly activate and inhibit the expression of ARF gene family.

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魏瑞敏,谢玲玲,欧阳娴,等.辣椒ARF基因家族的鉴定与表达分析[J].西北植物学报,2017,37(6):1047-1058

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  • 在线发布日期: 2017-07-17
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