外源钙对甘肃贝母倒苗特性和抗氧化酶活性的影响
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国家自然科学基金(31360316,31660158);


Effect of Exogenous Ca2+ on Sprout Tumble Characteristics and Antioxidant Enzyme Activities of Fritillaria przewalskii
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    摘要:

    对甘肃贝母(Fritillaria przewalskii Maxim.)鳞茎播种后2年生苗喷施不同浓度CaCl2溶液(5、10、15、20和25 mmol·L-1),以喷施等量蒸馏水为对照(CK),通过测定倒苗率变化和倒苗期地下部分生理特性,探讨外源钙对甘肃贝母倒苗特性的影响。结果显示:(1)外源CaCl2处理均可推迟甘肃贝母倒苗高峰期,有效延长甘肃贝母生长期,从而提高鳞茎产量;各CaCl2浓度处理的倒苗延迟效应基本表现为15 mmol·L-1>20 mmol·L-1>25 mmol·L-1>10 mmol·L-1>5 mmol·L-1> CK。(2)不同CaCl2溶液喷施后倒苗期较CK推迟效应显著,比CK倒苗高峰期推迟3~7 d,并推迟3~6 d结束倒苗高峰,其中以喷施15 mmol·L-1CaCl2处理的倒苗延迟效应最佳。(3)10~25 mmol·L-1CaCl2处理均可有效增强甘肃贝母倒苗期根系活力和鳞茎的抗氧化能力,其中以15 mmol·L-1 CaCl2处理的倒苗期根系TTC活力最高,鳞茎SOD、POD和CAT活性最强。(4)隶属函数法综合分析结果显示,不同浓度CaCl2处理下甘肃贝母倒苗期鳞茎抗性及生活力由强至弱依次为15 mmol·L-1>20 mmol·L-1>25 mmol·L-1>10 mmol·L-1>5 mmol·L-1> CK。研究表明,15 mmol·L-1 CaCl2为甘肃贝母延迟倒苗期、增强苗期鳞茎抗逆性、提高鳞茎产量的最佳处理。

    Abstract:

    In order to reveal the effect of exogenous Ca2+ on the characteristics of the sprout tumble, we sprayed the plant cultivated for two years with bulbs by different concentrations of CaCl2 solution (5, 10, 15, 20 and 25 mmol·L-1), using the equal distilled water as the control, then measured the changes of the rate for sprout tumble and the physiological characteristics of underground part on sprout tumble stage. The results showed that: (1) the different concentrations of exogenous Ca2+ had effect on the plant sprout tumble characteristics, effectively extending the growth period of Fritillaria przewalskii to increas the bulb yield. The effect of the concentration of CaCl2 on delaying the stage of sprout tumble was 15 mmol·L-1>20 mmol·L-1>25 mmol·L-1>10 mmol·L-1>5 mmol·L-1> CK. (2)The different concentrations of CaCl2 have significantly effected phenology of sprout tumble, and the greatest effect was found in the pretreatment sprayed by 15 mmol·L-1 CaCl2.Compared with the control, the phenology delayed 3~7 d at the fastigium of sprout tumble and 3~6 d to finish the fastigium of sprout tumble.(3)10~25 mmol·L-1CaCl2 can enhance the root TTC vigor and the activities of SOD,POD and CAT, and the bulbs have the highest levels of the root TTC vigor and activities of antioxidant enzymes by 15 mmol·L-1 CaCl2.(4)The results of comprehensive analysis of membership function show that, the resistance and viability of bulbs in F. przewalskii at sprout tumble stage by different concentrations of CaCl2 from strong to weak are 15 mmol·L-1>20 mmol·L-1>25 mmol·L-1>10 mmol·L-1>5 mmol·L-1> CK. All above suggest that 15 mmol·L-1 CaCl2 is the best treatment to delay phenology of sprout tumble,enhance the plant vitalities and the resistance, and increase bulbs yield.

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王小琴,陈 垣,郭凤霞,等.外源钙对甘肃贝母倒苗特性和抗氧化酶活性的影响[J].西北植物学报,2017,37(9):1831-1838

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  • 在线发布日期: 2017-10-23
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