The research was conducted for investigating the microspores development process and the abortive cytological characters of the male sterile plant A22013189 of Festuca arundinacea. With fertile plant 189 as control, we observed the seed production ability, pollen viability, meiotic chromosome behavior of pollen mother cells (PMCs), physiological and biochemical character.Results shows that: (1) the male sterile plant A22013189 could not get seeds by inbred with a spot of shriveled pollen grain. (2) Abnormal chromosome behaviors existed in PMCs from prophase I stage to tetrad stage, including a mass of lagging chromosomes, bridge fragments, micronucleus, univalent chromosomes, unequal separation, asynchronous division, free chromosomes, triad, triangle spindles and chromosome deletion, which were important cytological reasons resulted in abortive pollen of tall fescue. (3) There were obvious differences in physiological and biochemical characteristics of male sterile plant A22013189 and fertile plant 189 during all periods. The contents of soluble protein and soluble sugar during all periods of A22013189 were markedly lower than those of 189 the corresponding periods; from seedling stage to sporulation stage, there is no significant difference between the contents of free proline of A22013189 and 189, but during meiosis stage to pollen maturation stage, which are significantly higher than those of 189; from seedling stage to sporulation stage, the contents of malondialdehyde of A22013189 are markedly lower than those of 189. However, after the microspores development to meiosis stage, the increasing speed and contents of malondialdehyde of A22013189 are remarkably higher than male fertile 189. The research shows that abnormal chromosome behaviors, the reduction of substance and energy metabolism, accumulation of harmful substances are existed in male sterile plants development processes, which provided us important theoretical guiding significance about sterile mechanism research and crossbreeding parent selection of tall fescue.