接种AMF对弱光环境及盐胁迫下甜瓜光合特性的影响
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留学回国人员科技项目择优资助项目(2016);


Photosynthesis Responses of Cucumis melo Seedlings to Glomus under Low Light and Salt Stress
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    摘要:

    以甜瓜(Cucumis melo L.)品种“中蜜3号”为试材,摩西球囊霉菌(Glmous mosseae,GM)为供试菌种,采用温室盆栽试验研究了丛枝菌根真菌(Arbuscular Mycorrhizal Fungi,AMF)对弱光及盐胁迫下甜瓜幼苗叶绿素含量、光合气体交换参数、叶绿素荧光参数和光响应曲线的影响。结果表明:弱光及盐胁迫下接种AMF可显著增强甜瓜幼苗叶绿素含量、净光合速率(Pn)、光合电子传递速率(ETR)、水分利用率(WUE)和气孔限制因子(Ls);气孔导度(Gs)、蒸腾速率(Tr)、胞间CO2浓度(Ci)则随胁迫时间延长不断增强;光化学猝灭系数(qP)与实际光化学效率(ΦPSⅡ)变化趋势基本一致且均显著高于未接菌处理;接种AMF显著增强了最大光化学效率(Fv/Fm),但处理之间差异不显著;并进一步提高了宿主的最大羧化速率(Amax)、光饱和点(LSP)和表观量子效率(AQE),降低了光补偿点(LCP)和暗呼吸速率(Rd)。研究发现,AMF可通过改善叶绿素荧光、气体交换参数和光响应参数来减轻弱光及盐胁迫对植株造成的伤害,从而提高甜瓜对弱光及盐胁迫的耐性。

    Abstract:

    The effects of arbuscular mycorrhizal fungi (AMF) on the photosynthetic characteristics of melon under low light and salt stress were investigated by pot experiment in greenhouse. It follows that AMF significantly increased chlorophyll content, net photosynthetic rate (Pn), photosynthetic electron transport rate (ETR), water use efficiency (WUE) and stomatal limitation factor (Ls) in melon seedlings under low light and salt stress. Stomatal conductance (Gs), transpiration rate (Tr) and intercellular CO2 concentration (Ci) also increased with the prolongation of stress time. The changes of photochemical quenching coefficient (qP) and actual photochemical efficiency (ΦPSⅡ) were significantly higher than those of inoculation (Amax), light saturation point (LSP) and apparent quantum efficiency (AQE) significantly increased (P <0.05), and the maximum photochemical efficiency (Fv/Fm) was significantly enhanced by inoculation with AMF, but the difference was not significant, while light compensation point (LCP) and dark respiration rate (Rd) reduced. We propose that AM symbiosis can protect melon plants from low light and salt stress by improving their chlorophyll fluorescence, gas exchange parameters and light response parameters.

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黄 志,许炜萍,郁昉斌,等.接种AMF对弱光环境及盐胁迫下甜瓜光合特性的影响[J].西北植物学报,2018,38(2):307-315

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  • 在线发布日期: 2018-03-23
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