In order to get more information about the genetic background of agronomic and quality traits of flax (Linum usitatissimum L.) for marker assistant selection (MAS) and mapbased cloning, we constructed a F_2:3 QTL location population by using LH89 and R43 as parents and made a quantitative trait locus (QTL) location study for 13 agronomic and quality traits based on previously constructed linkage genetic map by composite interval mapping (CIM) method and R/QTL programme. The results are as follows: (1) 35 QTLs related to agronomic and quality traits in flax were detected. Five QTLs each for linoleic acid and oil content, four QTLs each for linoleic acid and thousand seeds weight, three QTLs each for palmitic acid, plant height, technical length, two QTLs each for stearic acid, branch number, one QTL each for bolls per plant, seeds per boll, seeds weight per plant and oleic acid. (2) Among them,18 QTLs which explained more than 10% of the phenotypic variation, i.e., eight for agronomic traits and ten for quality traits, suggesting an existence of majoreffect genes.