干旱胁迫对小紫珠光合生理的影响
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河北省林业科学研究院项目(17226320D);


Photosynthetic Characteristics of Callicarpa dichotoma under Drought Stress
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    摘要:

    以小紫珠(Callicarpa dichotoma)幼苗为试验材料,采用盆栽控水法设置4个水分梯度,分别为田间最大持水量的95%(对照)、75%(轻度干旱)、55%(中度干旱)和35%(重度干旱),研究土壤水分对小紫珠光合参数的影响。结果显示:(1)随着干旱胁迫的加剧,小紫珠的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和水分利用效率(WUE)逐渐下降,其胞间CO2浓度(Ci)在轻度和中度干旱胁迫下逐渐下降,在重度干旱胁迫下显著升高。(2)随着干旱胁迫的加剧小紫珠的光饱和点(LSP)、最大净光合速率(Pnmax)、暗呼吸速率(Rd)和表观量子效率(AQY)显著减少,光补偿点(LCP)极显著增加(P<0.01)。(3)随着胁迫程度的增加,小紫珠的最大荧光(Fm)、最大光化学效率(Fv/Fm)和PSⅡ的量子效率(Fv/Fo)逐渐减少,初始荧光(Fo)显著升高(P<0.01);随着干旱胁迫的加剧小紫珠能量分配比率参数φPo、Ψo、φEo逐渐下降,φDo逐渐增加;代表单位反应中心活性的参数ABS/RC、TRo/RC、ETo/RC和DIo/RC逐渐升高。(4)随着胁迫程度的增加,小紫珠叶绿素a、叶绿素b、叶绿素总量和叶绿素a/b逐渐减少。研究表明,干旱胁迫下小紫珠光合速率的降低既有气孔因素也有非气孔因素,但轻度和中度干旱胁迫下以气孔限制为主,而在重度干旱胁迫下以非气孔限制为主。

    Abstract:

    With Callicarpa dichotoma seedlings as test materials, using pot set four water moisture gradients, 95% of water holding capacity of the largest field (control), 75% (mild drought), 55% (moderate drought) and 35% (severe drought), we investgated the soil moisture affects C. dichotoma photosynthetic characteristics. The results show: (1) since the drought stress, the net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and water use efficiency (WUE) of C. dichotoma were decreased, while the intercellular CO2 concentration (Ci) in mild and moderate gradually decline, under severe drought stress significantly increased; (2) with the intensification of drought stress, the light saturation point (LSP), maximum net photosynthetic rate (Pnmax), dark respiration rate (Rd) and apparent quantum efficiency(AQY) of C. dichotoma significantly reduced, and light compensation point (LCP) increased significantly (P<0.01); (3) with the increase of degree of stress, the maximum fluorescence (Fm), maximum photochemical efficiency (Fv/Fm) and PSⅡ quantum efficiency (Fv/Fo) of C. dichotoma were gradually reduced and the initial fluorescence (Fo) was significantly increased (P<0.01), since the drought stress energy distribution ratio parameters, φPo, Ψo, φEo and DIo/RC are gradually increasing; (4) with the increase of stress, chlorophyll a, chlorophyll b, chlorophyll and chlorophyll a/b of C. dichotoma gradually decreased. The study showed that the decrease of the combined rate of C. dichotoma in the drought stress has both the stomatal factor and the nonstomatal factor. The photosynthetic rate of C. dichotoma under mild and moderate drought stress was dominated by stomatal restriction, while nonstomatal restriction was mainly due to severe drought stress.

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杨建伟,赵 丹,孙桂芳,等.干旱胁迫对小紫珠光合生理的影响[J].西北植物学报,2018,38(4):733-740

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  • 在线发布日期: 2018-05-21
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