遮光对地被菊花色苷含量和CmUFGT基因表达的影响
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国家“十二五”科技支撑计划(2013BAD01B07);


Effect of Shading on the Content of Anthocyanin and Expression of CmUFGT in Flowers of Groundcover Chrysanthemum
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    摘要:

    以地被菊品种‘紫重楼’为试材,设置4种光环境梯度:L0(CK,全光照)、L1(80%全光照)、L2(60%全光照)和L3(40%全光照),研究花序不同发育期遮光处理对‘紫重楼’开花过程中叶绿素含量、干重、干重比、花色苷含量、可溶性糖含量和CmUFGT基因表达量的影响,以探讨光在花色苷合成和降解过程中的作用。结果表明:(1)现蕾期、露色期遮光处理下,叶绿素a、b含量随光照强度的降低而逐渐增加,其中L3处理下叶绿素a、b含量显著高于其他处理;盛花期遮光处理下,叶绿素a、b含量随光照强度的降低呈先增后降的趋势,其中L1处理下叶绿素a、b含量极显著高于其他处理。(2)花序干重随着光照强度的降低呈下降趋势,花序干重比呈先升后降的趋势,其中L1处理下花序干重比增加。(3)现蕾期、露色期遮光处理下花色苷含量随着花序发育呈先升后降的变化趋势,盛花期遮光处理下呈下降趋势;花序发育第3、4阶段,光照越强花色苷含量越高;第7、8阶段,光照越弱花色苷含量越高。(4)遮光处理下舌状花可溶性糖含量随着花序发育呈先升后降趋势,其中L1处理下可溶性糖含量增加。(5)遮光处理极显著抑制花序发育第5阶段CmUFGT基因的表达,且表达量随着光照强度的降低而逐渐降低;现蕾期、露色期L1处理下,花序发育第3、4阶段CmUFGT基因表达量降低。研究表明,地被菊花序发育中期(第3、4阶段)轻度遮光(L1)抑制CmUFGT基因表达,促进可溶性糖含量增加,有利于花色苷的合成;花序发育后期(第7、8阶段)重度遮光(L3)有利于缓解花色苷的降解。

    Abstract:

    The effects of shading on the chlorophyll content, dry weight, dry weight ratio anthocyanin content, soluble sugar content and CmUFGT expression level in flowers were studied with Chrysanthemum cultivars ‘Zichonglou’ at different flower developmental stages. The results showed that: (1) shading at visible flower bud stage and visible flower color stage, Chl a and Chl b contents increased gradually with the decrease of light intensity; Chl a and Chl b contents of L3 treatment were significantly higher than that of other treatments. While Chl a and Chl b contents were first rose and then dropped with the decrease of light intensity under shading at fullbloom stage; Chl a and Chl b contents of L1 treatment were significantly higher than that of other treatments. (2) With the decrease of light intensity, the dry weight of inflorescence decreased, and the dry weight ratio increased firstly and then decreased; The dry weight ratio of L1 treatment increased. (3) Shading at visible flower bud stage and visible flower color stage, the anthocyanin content was increased firstly and then decreased following flower inflorescence development, while the anthocyanin content was decreased under shading at fullbloom stage; In the third and fourth stages of inflorescence development, anthocyanin content increased when the light increased; In the seventh and eighth stages, anthocyanin content was increased when the light decreased. (4) Following flower inflorescence development, the content of soluble sugar under shading increased initially and then decreased; The content of soluble sugar increased in L1 treatment. (5) Shading significantly inhibited the expression of CmUFGT at the fifth stage of inflorescence development, and its expression decreased gradually with the decrease of light intensity; The expression of CmUFGT reduced in the third and fourth stages in L1 treatment at visible flower bud stage and visible flower color stage. It has been concluded that following the flower inflorescence development under the mild shading (L1) inhibits the expression of CmUFGT, promotes the increase of soluble sugar content and the synthesis of anthocyanin at middle stages (the third, fourth stage); the severe shading (L3) alleviates the degradation of anthocyanin at the late stages (the seventh, eighth stage).

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杨中义,尚嘉琪,赵夏陆,等.遮光对地被菊花色苷含量和CmUFGT基因表达的影响[J].西北植物学报,2018,38(6):1109-1117

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  • 在线发布日期: 2018-07-11
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