In this study, the new species Magnolia wufengensis ‘Jiaohong No.1’ was selected as the research material in Wufeng, Hubei. A SUPERMAN like gene MwZFP10 (GenBank accession MH037314) was cloned with 3′RACE method, based on the 645 bp core fragment related with floral development and the fragment was filtered out from differentially expressed genes based on preLaboratory cDNA library of Magnolia wufengensis. Bioinformatics analysis of MwZFP10 gene showed that the full length of MwZFP10 cDNA was 1 117 bp with an open reading frame (ORF) of 726 bp encoding 241 amino acids. The protein molecular formula is C₁₁₂₈H₁₇₆₀N₃₂₂O₃₈₃S₁₄, molecular weight is 26.4 kD and isoelectric point is 4.42. The protein contains a conserved domain: the C2H2type zinc finger. Comparative analysis found that MwZFP10 belongs to the SUPERMANlike zinc finger protein, with the “QALGGH” in the N terminal and “L(I)DLXLR(K)L” in the C terminal. αhelix (Hh), extended chain (Ee) and βturn (Bt) constitute are the basic structure of the protein. The tertiary structure of the protein has a typical zinc finger spatial configuration. The protein is hydrophilic and mainly localized in the nucleus. The semiquantitative PCR and quantitative realtime PCR results showed that MwZFP10 was expressed in floral organs, leaf buds and bracts, and the pistil expression was higher in floral organs. MwZFP10 transgenic Arabidopsis thaliana is characterized by short and sterile plants, curling leaves, malformation of flower organs, etc. The gene MwZFP10 play an important role in the floral growth and development.