In this study, we cloned a dehydrin encoding gene LcDHN3 from Leymus chinensis. The sequence analysis shows that the open reading frame of LcDHN3 is 501 bp and encodes 167 amino acids, molecular weight is 17.01 kD, theoretical pI is 8.05, contains dehydrin superfamily domain, and one Ysegment, one Ssegment and two Ksegments, belongs to YSK2 type dehydrin. Subcellular localization predicts LcDHN3 may localized in both cytoplasm and nuclei. Multiple alignment of homology sequences showed that the identity of LcDHN3 with DHNs from other plants was 84.27%. Phylogenetic analysis revealed LcDHN3 had the closest genetic relationship with Hordeum vulgare DHN. The transcripts of LcDHN3 were induced by different abiotic stresses, including drought, cold, heat, NaCl, high pH and wounding, as well as ABA and JA treatments. These results indicate that LcDHN3 involved in the signal transduction pathway of L. chinensis in response to abiotic stresses.