Epimedium pubescens Maxim. is a species widely distributed in China and abundant in morphological variation. There are still many voices in the classification of E. pubescens and its related species. This study chose 31 populations of E.pubescens Maxim.and its related species including 3 populations of E.stellulatum Stearn, 1 population of E.zhenbaense sp.nov. and 1 population of E.wanyuanense sp.nov. and observed nonglandular hairs on the back of their leaves, so as to study the variation law of E.pubescens and its related species and their intraspecific division. The results are as follows. (1) the nonglandular hairs from what we saw are divided into 7 large classes and 19 small classes, namely straight hair, curved hair, appressed curly hair, long appressed hair, pseudo short appressed hair, short appressed hair and single cell hair, and found that each population or individual was composed of many types of nonglandular hairs. (2) Mastoid were found in 13 populations and the population which has nonglandular hair under 50/mm², accounting 69.2%, and 27 populations has single cell hair, accounting 75%. (3) Based on nonglandular hair density and major types, we come to conclusion of 36 populations classification. 21 populations of E.pubescens are straight hair group (A) and 7 populations are curly hair group (B). Long appressed hair group (D) contains 2 populations of E.pubescens, 3 population of E.stellulatum and 1 population of E.zhenbaense. One population of E.pubescens is pseudo short appressed hair group (E) and one population of E.wanyuanense is short appressed hair group (F). All populations have straight hair, showing that the core type in E.pubescens and its related species is straight hair. (4) From the angle of nonglandular hairs type, the evolutionary relationship between populations is combed, namely is AⅠ→AⅡ→AⅢ→AⅣ→AⅤ, BⅠ→BⅡ→BⅢ→BⅣ, DⅠ→DⅡ→DⅢ, straight hair group→curly hair group, short straight hair group→short appressed hair or pseudo short appressed hair, and it shows a good correlation with geographical distribution as well. (5) Single cell hair has been found for the first time in Epimedium and considered to have some relation to the main hair type (straight hair and curly hair) and geographical distribution. However, there is a negative correlation between the mastoid and nonglandular hairs density on back of the leaves. The results of this study support the classification treatment that E.stellulatum Stearn is under the species of E. pubescens Maxim.