Gammatocopherol methyltransferase (γTMT) is one of the key enzymes in the vitamin E synthesis pathway. In this study, we cloned three homologous copies of the γtocopherol methyltransferase gene (γTMT) from the wheat cultivarChinese Spring using RTPCR (reverse transcription PCR). The similarity of three sequence CDs (coding sequence) was 99.12%. TaγTMT6A and TaγTMT6B coding sequences both contain 1 101 bp and encode 366 amino acids, while TaγTMT6D encodes only 365 amino acids due to nonsense mutations. The results of bioinformatics analysis showed that the molecular weights of the three proteins were 39.58, 39.53 and 39.50 kDa, respectively, and the theoretical isoelectric points were 6.67, 6.41 and 7.08. They all belonged to hydrophilic unstable nonsecretory proteins. Phylogenetic tree analysis indicated that TaγTMT protein shared high similarity to γTMT protein from Panicum miliaceum and closest genetic relationship. Realtime quantitative PCR analysis showed that the TaγTMT gene was expressed in roots, stems, leaves, glumes and seeds, and highly expressed in leaves. Meanwhile, expression of the TaγTMT gene was upregulated induced by ABA, NaCl, PEG treatments. This result will provide the foundation for further study of the function of the TaγTMT gene in wheat.