The EPSPS gene which encodes 5enolpyruvylshikimate3phosphate synthase, the key enzyme of aromatic amino acid synthesis has been cloned and widely studied in bacteria, fungi, algae, and plants. EPSPS is the target enzyme of glyphosate herbicide and its overexpression can improve glyphosate resistance of crops. In this study, primers were designed according to the genomic database of sweet potato, RNA was extracted from the sweet potato variety, ‘Guangshu 87’, and the gene amplified by RTPCR. After sequencing, bioinformatic analysis and expression analysis were carried out. The results showed that: (1) the total CDs of the IbEPSPS gene was 1 569 bp, encoding 522 amino acids, among these, there were two conserved domains of IbEPSPS gene in amino acid sequence 98-113 and 173-183 positions. (2) Phylogenetic tree analysis showed that the EPSPS of sweet potato was closely related to Ipomoea triloba, Calystegia hederacea, Convolvulus arvensis, and Ipomoea nil. (3) The results of realtime quantitative PCR showed that the IbEPSPS gene was highly expressed in the stem, leaf and shoot tip of sweet potato, and the expression of the IbEPSPS gene increased after the stress of glyphosate. The results provide a reference for further study on the function of the IbEPSPS gene and the mechanism of resistance of sweet potato to glyphosate.