小麦萌发期抗旱和耐盐性状的QTL分析
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山东省重大科技创新工程(2018YFJH0602);


QTL Mapping of Drought and Salt Tolerant Traits in Wheat at Germination Stage
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    摘要:

    该研究以‘山农0431×鲁麦21’RIL群体及其父母本为材料,用20% PEG6000溶液和100 mmol·L-1 NaCl溶液分别模拟干旱和盐环境,对12个小麦萌发期抗旱耐盐相关性状进行测定,结合已构建的分子标记遗传图谱对小麦萌发期抗旱、耐盐的相关性状进行QTL分析,为小麦抗旱、耐盐基因的克隆和分子标记辅助选择提供参考。结果表明:(1)正常、干旱和盐胁迫3种处理下共检测到143个QTL。检测到相对高频QTL(RHFQTL)29个,平均贡献率范围为4.39%~13.28%,贡献率在10%以上的主效RHFQTL有10个。(2)检测到胁迫下特异表达的RHFQTL共17个,正常处理下特异表达的RHFQTL为8个,稳定表达的RHFQTL为4个。(3)QTL分析结果表明,7个RHFQTL形成了3个QTL簇,且分布在2D、4D和5B等3条染色体上,其中:QC1位于2D染色体的wPt6847~D1172783区间,包括3个QTL(QRl2D.2、QSdw2D.3、QTdw2D);QC2位于4D染色体短臂的D2245724~D1108531区间,包括2个QTL(QSl4DQShl4D);QC3位于5B染色体的D982263~S1083095区间,包括2个QTL(QSl5B.2、QTdw5B.1)。

    Abstract:

    In this study, the test material was carried out using RILs (recombinant inbred lines) derived from the cross of ‘Shannong 0431 × Lumai 21’. Drought and salt environments were simulated with 20% PEG6000 solution and 100 mmol·L-1 NaCl, respectively. We analyzed quantitative trait loci (QTL) for drought and salt tolerant related traits in wheat on the 12 drought and salt tolerant traits in wheat germination, combining with a genetic map constructed by our group, to provide references for the cloning of salttolerant and droughtresistant genes and markerassisted selection of wheat. The results showed that: (1) a total of 143 QTLs were detected for 12 traits under normal, drought and salt environments. A number of 29 RHFQTLs were detected with the average contribution rate ranged from 4.39% to 13.28%. Among them, the contribution rate of 10 RHFQTLs was more than 10%. (2) A total of 17 and 8 RHFQTLs with specific expression were detected under stress and normal treatments, respectively. Four RHFQTLs with stable expression were detected. (3) A number of three QTL clusters formed by 7 RHFQTLs were detected, distributing on chromosomes 2D, 4D and 5B. Among them, QC1 was located in the wPt6847-D1172783 of 2D chromosome, including three QTLs (QRl2D.2, QSdw2D.3, QTdw2D). QC2 was located in the D2245724-D1108531 of 4D chromosome, including two QTLS (QSl4D, QShl4D). QC3 is located in D982263-S1083095 of chromosome 5B chromosome, including two QTLS (QSl5B.2, QTdw5B.1).

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王秀华,于鸿翔,孙福来,等.小麦萌发期抗旱和耐盐性状的QTL分析[J].西北植物学报,2020,40(2):240-251

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  • 在线发布日期: 2020-05-02
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