光氮互作对闽楠幼苗叶片光合生理特性的影响
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国家自然科学基金(31360174);


Effect of Light and Nitrogen Interaction on Photosynthetic Physiological Characteristics in Leaves of Phoebe bournei Seedlings
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    摘要:

    以5月龄闽楠幼苗为材料,采用4种光照水平(100%、41.3%、14.3%、3.6%自然光)和4种氮素水平(0、0.5、1、2 mol/L纯氮)的双因素盆栽试验,研究光氮互作下闽楠幼苗的光合生理特性,以探讨其对环境适应性的生理机制。结果表明:(1)遮荫和施氮均能显著提高闽楠叶片光合色素含量;在同一氮素水平下,随光照强度下降,闽楠幼苗叶片可溶性糖(SS)、可溶性蛋白(SP)、净光合速率(Pn)、气孔导度(Gs)、最大净光合速率(Pmax)、暗呼吸速率(Rd)、表观量子系数(AQY)均呈先增后降趋势,并在41.3%自然光照水平时最高;而同期叶片胞间CO2浓度(Ci)、蒸腾速率(Tr)、光饱和点(LSP)、光补偿点(LCP)均呈下降趋势;闽楠幼苗的叶片PSⅡ最大光化学效率(Fv/Fm)和PSⅡ潜在活性(Fv/Fo)则随光照减弱逐渐增加。(2)同一光照水平下,随着氮素施用量增加,闽楠幼苗叶片SS、SP、光合气体交换参数、光响应曲线特征参数总体呈现先升高后降低的趋势,Fv/FmFv/Fo呈先降后增趋势。(3)不同光照和氮素水平之间存在显著的互作效应,隶属函数分析结果以41.3%自然光照、0.5 mol/L纯N处理的效果最优。研究表明,闽楠幼苗为喜光耐荫植物;适宜遮荫和施氮处理组合可显著改善闽楠渗透调节能力,提高光能利用率,促进光合作用进行,而全光照、过度遮荫、缺氮、或过量施氮均不利于闽楠正常生理代谢。

    Abstract:

    We conducted a pot experiment using 5monthold Phoebe bournei seedlings with twofactor including four light treatments (100%, 41.3%, 14.3%, 3.6% Nature Source light) and four nitrogen levels (0, 0.5, 1, 2 mol/L) to study the photosynthetic physiological characteristics of Phoebe bournei under the interaction of light and nitrogen, to explore the physiological mechanism of its adaptability to the environment. The results showed that: (1) shading and nitrogen could significantly improve the photosynthetic pigment content of P. bournei. With the decrease of light intensity, the soluble sugar (SS), soluble protein (SP), net photosynthetic rate (Pn), stomatal conductance (Gs), maximum net photosynthetic rate (Pmax), dark respiration rate (Rd), apparent quantum coefficient (AQY) in leaves of P. bournei seedlings increased firstly and then decreased under the same nitrogen, and the highest was at 41.3% natural light level. The intercellular CO2 concentration (Ci), transpiration rate (Tr), light saturation point (LSP) and light compensation point (LCP) decreased at the same term. The maximum photochemical efficiency (Fv/Fm) and potential activity (Fv/Fo) of PSⅡ increased with the decrease of light intensity. (2) With the increase of nitrogen application, SS, SP, photosynthetic gas exchange parameters and characteristic parameters of light response curve of P. bournei leaves increased firstly and then decreased, Fv/Fm and Fv/Fo decreased then ascend. (3) There was a significant interaction between different light and nitrogen levels. Membership function analysis results showed that 41.3% natural light and 0.5 mol/L pure N treatment had the best effect, the research indicated that the P. bournei seedlings were photophilous and shade plant, and the combination of suitable shading and nitrogen application can significantly improve the osmotic adjustment ability, improve the utilization rate of light energy, and promote photosynthesis of P. bournei, while full light, excessive shading, nitrogen deficiency, or excessive nitrogen application were not conducive to the normal physiological metabolism of P. bournei.

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施福军,粟春青,韦 艺,等.光氮互作对闽楠幼苗叶片光合生理特性的影响[J].西北植物学报,2020,40(4):667-675

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  • 在线发布日期: 2020-06-22
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