山丹百合鳞茎提取液中活性物质及其对樱桃番茄的保鲜效果
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宁夏回族自治区重点研发计划项目(2017BY071);


Active Substances in Bulb Extracts from Lilium pumilum and Its Preservation for Cherry Tomato
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    摘要:

    以山丹百合鳞茎和樱桃番茄‘碧姣’果实为材料,分析不同浓度(0~1.0 g/L,以不做任何处理为对照CK)百合鳞茎提取液在低温(5 ℃)和常温(20 ℃)贮藏期间对樱桃番茄果实感观品质、营养品质和超氧化物歧化酶(SOD)活性的影响,并采用比色法、比浊法等方法测定提取液中活性成分含量及其抗氧化能力、抑菌活性,探究山丹百合鳞茎浸提液对采后樱桃番茄果实的保鲜效果及其机理。结果表明:(1)用0~1.0 g/L百合鳞茎提取液处理樱桃番茄果实,在常温和低温下果实分别可贮藏21 d和35 d,贮藏期分别比CK延长了3~6 d和7~14 d。(2)低温下适宜浓度(0.4~0.8 g/L)和常温下各浓度百合鳞茎提取液均能有效抑制番茄果实硬度下降,延缓质量损失率和色度的升高,而在常温和低温下均以0.8 g/L处理效果最佳;在贮藏结束时,常温(低温)贮藏的质量损失率和色度分别比CK显著降低了70.27%(58.82%)和93.44%(89.33%),硬度显著提高了81.11%(69.78%)。(3)低温下适宜浓度和常温下各浓度百合鳞茎提取液均能显著延缓番茄果实维生素C(Vc)和总酸(TA)的消耗,以及可溶性固形物(TSS)含量的升高,而在常温和低温下仍以0.8 g/L处理效果最佳;在贮藏结束时,常温(低温)贮藏果实TA和Vc含量分别比CK显著提高了65.59%(65.06%)和70.87%(53.44%),而TSS含量显著降低了93.49%(84.62%)。(4)低温下适宜浓度和常温下各浓度的百合鳞茎提取液能有效延缓番茄果实SOD活性的下降,而在常温和低温下均以0.8 g/L处理效果最佳,贮藏结束时,常温和低温下果实SOD活性分别比CK显著提高了66.42%和70.80%。(5)0.8 g/L百合鳞茎提取液中富含多糖、黄酮、黄烷醇、皂甙、总酚和生物碱等活性成分(0.34~2.41 mg/g),对DPPH自由基清除力和铜离子还原能力分别达到7.10和1.06 μmol/g,并对番茄青霉菌生长具有明显的抑制作用,青霉菌数量较对照组显著下降了74.44%~79.76%。研究发现,百合鳞茎提取液中富含多糖、皂甙和多酚等活性成分,具有良好的抗氧化能力和抑菌活性;适宜浓度的百合鳞茎提取液能有效降低番茄果实质量损失率,保持番茄硬度和色度,维持维生素C、总酸和可溶性固形物等营养成分,并保持较高的SOD活性,明显延长贮藏时间,表现出良好的保鲜效果,并以0.8 g/L浓度的百合鳞茎提取液的保鲜效果最佳。

    Abstract:

    We used Lilium pumilum bulbs and cherry tomato (Lycopersicon esculentum var. cerasiforme) as the experimental materials to analyze the effects of L. pumilum bulb extracts in different concentrations (0~1.0 g/L, without any treatment for the control group) on fruit sensory quality, nutritional quality and superoxide dismutase (SOD) activity during storage at low temperature (5 ℃) and room temperature (20 ℃). A colorimetric method combined with a turbidimetric method was employed to determine the contents of active ingredients in the L. pumilum bulb extracts and the antioxidant capacity as well as antibacterial activity, and to investigate the preservation effects of the L. pumilum bulb extracts on cherry tomato. Results revealed as follows: (1) with 0-1.0 g/L L. pumilum bulb extracts soaking cherry tomato, then cherry tomatoes were stored at 5 ℃ for 21 d and at 20 ℃ for 35 d, respectively, and accordingly under such circumstances the storage periods were 3~6 d and 7~14 d, respectively, longer than that of the control group. (2) The L. pumilum bulb extracts both in the appropriate concentration (0.4~0.8 g/L) at 5 ℃ and in various concentrations at 20 ℃ inhibited the decrease in cherry tomato firmness effectively, delayed the increase in mass loss rate and chroma, and additionally both at 20 ℃ and at 5 ℃, the effects of 0.8 g/L were the best. At the end of storage, mass loss rate and chroma at 20 ℃ (5 ℃) storage temperatures were significantly lower than those of the control by 70.27% (58.82%) and 93.44% (89.33%), while the firmness at both storage temperatures was significantly increased by 81.11% (69.78%). (3) Both the L. pumilum bulb extracts in the appropriate concentration at 5 ℃ and those in various concentrations at 20 ℃ significantly delayed the consumption of vitamin C (Vc) and total acid (TA) in cherry tomato, and the increase in soluble solids (TSS) content, while at 20 ℃ and at 5 ℃, the effects of 0.8 g/L were the best. At the end of storage, the contents of TA and Vc in cherry tomato stored at 20 ℃ (5 ℃) were significantly higher than that of the control by 65.59% (65.06%) and 70.87% (53.44%), while the content of TSS was significantly reduced by 93.49% (84.62%). (4) The L. pumilum bulb extracts in the appropriate concentration at 5 ℃ and those in various concentrations at 20 ℃ effectively delayed the decrease of SOD activity, while at 20 ℃ and at 5 ℃, the effects of 0.8 g/L were the best. At the end of storage, the SOD activity in cherry tomato at 20 ℃ (5 ℃) were significantly increased by 66.42% and 70.80%, respectively in relation to that of the control. (5) The 0.8 g/L lily bulb extracts were rich in polysaccharides, flavonoids, flavanols, saponins, total phenols, alkaloids and other active ingredients (0.34~2.41 mg/g), which had the scavenging capacity up to 7.10 μmol/g and 1.06 μmol/g for DPPH and copper ion, respectively, and the significant inhibitory effect on the growth of Penicillium on tomato. The number of Penicillium was effectively lower than that of the control by 74.44%~79.76%. The present study demonstrated that the extracts of L. pumilum bulbs were rich in polysaccharides, saponins, polyphenols and other active ingredients, which had efficient antioxidant and antibacterial activities. Also, the appropriate concentration of L. pumilum bulb extracts could effectively reduce the mass loss rate of cherry tomato, maintain the firmness and chroma of cherry tomato, preserve the nutritional components such as vitamin C, total acid and soluble solids, and prolong storage time evidently, and exhibit good freshness preservation effects, with the concentration of L. pumilum bulb extracts at 0.8 g/L having the best freshkeeping effect.

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杨 英,张 萍,葛蓓蕾,等.山丹百合鳞茎提取液中活性物质及其对樱桃番茄的保鲜效果[J].西北植物学报,2021,41(5):795-807

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  • 在线发布日期: 2021-06-22
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