In order to reveal the function of NAC gene in pepper, we cloned the fulllength gDNA sequence and cDNA sequence of CaNAC55 gene with pepper CM334 as the experimental material. Bioinformatics analysis showed that the full length of CaNAC55 gDNA was 4 164 bp, the full length of CaNAC55 cDNA and the complete open reading frame (ORF) were 1 299 bp, and the protein encoded by the gene was composed of 432 amino acid residues. The results of gene sequence alignment and homology analysis showed that CaNAC55 shared 99.87%, 93.37% and 92.62% homology with Capsicum annuum (XM016722474), Solanum lycopersicum (XM004241285) and Solanum tuberosum (XM006361027) respectively. Realtime fluorescence quantification showed that drought, heat shock and high salt could induce the expression of CaNAC55 gene and the expression of CaNAC55 gene reached the peak at 24 h, 24 h and 12 h after drought, high salt and heat shock treatment, which were 3.01, 20.92 and 8.84 times of the control, respectively. The relative expression of CaNAC55 gene in ABA treatment was significantly lower than that in control, indicating that the expression of CaNAC55 gene was inhibited by ABA. The results showed that the response of CaNAC55 transcription factor to different stresses was different. It was speculated that CaNAC55 gene might be an important regulator in response to stress in C. annuum.