盐碱胁迫下香椿幼苗光合及抗逆生理特性
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山东省农业科技资金林业科技创新项目(2019LY005)


Photosynthetic Characteristics and Stress Tolerance Physiology of Toona sinensis Seedlings under Salinealkali Stress
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    摘要:

    将1年生香椿实生苗进行盆栽,设置A(pH=7.16)、A(pH=8.47)、A(pH=9.91) 3个碱水平,每个碱水平下设S50(50 mmol/L)、S100(100 mmol/L)、S150(150 mmol/L)、S200(200 mmol/L) 4个盐浓度,测定不同处理下香椿叶片光合参数、叶绿素含量、膜损伤程度、保护酶活性和渗透调节物质含量等指标,探究盐碱胁迫对香椿幼苗光合及生理生化特征的影响,为盐碱地香椿抗逆栽培提供理论依据。结果表明:(1)在AS50和AS100处理下,香椿幼苗地上部整体长势良好。(2)随着盐碱浓度增加,香椿叶片净光合速率、蒸腾速率、气孔导度等光合参数和叶绿素含量总体呈下降趋势,而胞间二氧化碳浓度呈上升趋势。其中,在S50和S100盐浓度处理下,A碱水平处理香椿幼苗的各光合指标与对照相比无显著差异(P>0.05),A和A处理时幼苗叶片光合效率有所下降;而较高盐浓度处理时(S150和S200)各碱水平下叶片光能利用率均受到显著抑制。(3)随盐碱胁迫的加剧,叶片相对电导率、丙二醛及脯氨酸含量均显著上升,其SOD活性以及可溶性蛋白和可溶性糖含量则先升后降。研究认为,香椿幼苗对碱胁迫的敏感性比盐胁迫更强,土壤pH值是影响香椿幼苗生长的重要因素;盐碱混合胁迫显著抑制了香椿幼苗叶片光合作用,但香椿可以通过调控抗氧化系统和渗透调节物质来适应盐碱胁迫环境,从而具备一定的耐盐碱能力。

    Abstract:

    To reveal the effects of salinealkali stress on Toona sinensis seedlings, we treated the potted 1yearold seedlings of Toona sinensis with three alkali levels, A (pH=7.16), A (pH=8.47), A (pH=9.91), and four salt concentrations, S50(50 mmol/L), S100(100 mmol/L), S150(150 mmol/L), S200(200 mmol/L). The photosynthetic parameters, chlorophyll content, membrane damage degree, protective enzyme activity and osmotic regulation substance content under different treatments were examined to provide theoretical basis for developing cultivation of Toona sinensis on saltalkali lands. The results showed that: (1) under A S50 and A S100 treatments, the aboveground part of Toona sinensis seedlings grew well. (2) With the increases of salt and alkali stress concentration, net photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll content of Toona sinensis leaves were decreased, while the intercellular carbon dioxide concentration appeared increasing. Under the salt concentration of S50 and S100, the photosynthetic index of Toona sinensis seedlings treated with A alkali level did not show significant differences from that of the control (P > 0.05), while the photosynthetic efficiency of seedling leaves decreased significantly under A and A levels. Under the salt concentrations of S150 and S200, each level of alkali treatment significantly inhibited the photosynthetic efficiency in leaves. (3) With the increased salinealkali stress, the relative conductivity, the content of malondialdehyde and the content of proline increased, while the SOD activity, the contents of soluble protein and soluble sugar demonstrated early increase and late decrease. So, the seedlings of Toona sinensis were more sensitive to alkali stress than salt stress, and soil pH is an important factor affecting seedling growth of Toona sinensis. Salinealkali stress significantly inhibited the leaf photosynthesis of Toona sinensis seedlings. However, Toona sinensis can adapt to the salinealkali stress environment by regulating the antioxidant system and osmotic regulation substances, so that it has a certain tolerance to salinealkali conditions.

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巩志勇,辛建华,商小雨,等.盐碱胁迫下香椿幼苗光合及抗逆生理特性[J].西北植物学报,2021,41(7):1199-1209

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  • 在线发布日期: 2021-08-19
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