The seedling male sterile plants "Y-14" and "Sunshine Muscat" were used as test materials to study the changes of antioxidant enzyme activity and endogenous hormone content during the bud development period, further demonstrating the physiological and biochemical characteristics of new germplasm about male sterility of in grapes. The results showed that: (1)compared with "Sunshine Muscat", the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the flower buds of "Y-14" were significantly reduced, while the content of malondialdehyde(MDA) increased significantly;(2) the content of auxin (IAA) of in "Y-14" was higher than that in "Sunshine Muscat", while the content of abscisic acid (ABA) was significantly lower than "Sunshine Muscat"; In the early stage of flower bud development, the content of gibberellin (GA3) in "Y-14" is lower than "Sunshine Muscat", while methyl jasmonate (MeJA) is significantly lower than "Sunshine Muscat" in the later stage; the content of in "Y-14" trans-Zeatin Nucleoside (TZR) is lower than that in "Sunshine Muscat".(3) In the early mononuclear stage, the content of ABA of in "Y-14" changed smoothly while the "Sunshine Muscat" increased sharply. At the same time, the content of GA3 began to be higher than that in "Sunshine Muscat", while the MeJA began to be lower than "Sunshine Muscat" in the early mononuclear stage. And the difference is gradually significantit showed gradually significant difference. [Conclusion] So,The abnormal changes of SOD, POD, CAT activity and MDA, IAA, ABA, GA3, MeJA in the bud development stage of the sterile seedlings of "Sunshine Muscat" may have led to the male sterility. The early mononucleus period may be the critical period for the male sterility of "Y-14".