Using Cymbidium faberi and Cymbidium sinense as experimental material, we cloned AGAMOUS (AG) genes by RTPCR, and the tissue expression was studied by realtime fluorescence quantitative PCR (qRTPCR). It was found that: (1) three AG genes were obtained, they belong to MIKC MADSbox gene, and the two AG genes of C. faberi were named as CfAG1 (accession number: MW654188) and CfAG2 (accession number: MW654189). The AG gene of C. sinense was named as CsAG1 (accession number: MW654190). (2) CfAG1 was highly expressed in the gynostemium at fullblossom stage, and moderately expressed in buds at bud stage and ovaries at fullblossom stage. CfAG2 was highly expressed in ovary at fullblossom stage, moderately expressed in gynostemium at fullblossom stage, and slightly expressed in buds at bud stage and petals (including lip petals) at fullblossom stage. The relative expression of CsAG1 gene was the highest in the gynostemium at fullblossom stage, followed by buds at bud stage and ovary at fullblossom stage, and the lowest expression was in sepals and leaves at fullblossom stage. The study indicated that the expression characteristics of CfAG1 and CsAG1 were similar. These three genes are tissuespecific and can regulate the development of gynostemium and ovary. The results provided basic data and scientific basis for the following research on the development and evolution of floral pattern, molecular breeding and new variety breeding of Cymbidium.