百合LbCATLbGPX基因的克隆与表达分析
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国家自然科学基金(31760588)


Cloning and Expression Analysis of LbCAT and LbGPX in Lilium
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    摘要:

    以切花百合(Lilium brownii var. viridulum)‘卡瓦纳’cDNA为模板,克隆了过氧化氢酶(LbCAT)和谷胱甘肽过氧化物酶(LbGPX)基因。序列分析表明,这2个基因分别包含1 479 bp和519 bp的开放阅读框(ORF),编码492个和172个氨基酸。进化分析结果表明,LbCAT蛋白与岷江百合CAT蛋白的氨基酸序列相似性最高(99.19%),且亲缘关系最近;LbGPX蛋白与油棕GPX蛋白的氨基酸序列相似性最高(78.61%),亲缘关系最近。qRTPCR结果显示,LbCATLbGPX在百合根、鳞茎、叶和花中都有表达。LbCAT在叶中表达量最高,LbGPX在花中表达量最高。这2个基因在百合花蕾的生长发育过程中均有表达,且表达量逐渐增加;在PEG处理后2个基因的转录水平升高,但独角金内酯(SLs)处理却显著降低了这2个基因的转录水平;该结果为百合抗逆性机理研究以及抗逆育种奠定了基础。

    Abstract:

    Catalase gene (LbCAT) and glutathione peroxidase gene (LbGPX) were cloned from cutting lily (Lilium brownii var. viridulum)‘Corvara’cDNA. Sequence analysis showed that the two genes contained 1 479 bp and 519 bp, the opening reading frames (ORFs) were 492 and 172 amino acids, respectively. The evolutionary analysis showed that the amino acid sequences of LbCAT protein and CAT protein of Lilium regale had the highest consistency (99.19%) and the closest genetic relationship. The amino acid sequences of LbGPX protein and oil palm GPX protein had the highest consistency (78.61%) and the closest genetic relationship. The results of expression showed that LbCAT and LbGPX genes were transcribed in roots, bulbs, leaves and flowers. The leaves had the highest transcript profile of LbCAT and the flowers had the highest expression levels of LbGPX. These two genes were transcribed in different stages of lily buds and the expression levels increased following the buds growing . After PEG treatments, the transcription levels of the two genes surged, while the SLs treatments significantly lowed the two genes expression levels. These results provided a certain basis for the study and breeding of lily stress resistance.

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迟博文,刘 立,谢 天,等.百合LbCATLbGPX基因的克隆与表达分析[J].西北植物学报,2021,41(9):1467-1474

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  • 在线发布日期: 2021-09-28
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