The Pht1 family of phosphate transporters mediate the uptake and remobilization of phosphorus (P) in plants. To investigate the structure and expression pattern of the Pht1 (GuPht1) gene family in Glycyrrhiza uralensis, this study analyzed the GuPht1 gene family by using bioinformatic approaches. The spatial and temporal expression of GuPht1 under abiotic stress was analyzed by qRTPCR and transcriptome data. And four genes were cloned by reverse transcription PCR. The results showed that: (1) there were a total of 8 Pht1 genes (named GuPht1;1—GuPht1;8) in G. uralensis, all of which located on the cell membrane and had 12 transmembrane structures, belonging to the MFS superfamily. The length of amino acid sequence ranged from 521 to 570 aa, and contained a conserved Pht1 characteristic sequence (GGDYPLSATIMSE). (2) In phylogenetic analysis, the members of the GuPht1 gene family were closely related to those of legumes. There were some elements, such as Wbox, Gbox, PHOlike and P1BS in the promoter regions with functions in P starvation. The GuPht1 were evenly distributed on the scaffold, and GuPht1 proteins were monomeric proteins. (3) Transcriptome analysis showed that the GuPht1 played a certain role in response to drought and salt stresses, and had tissuespecific expression pattern. GuPht1;1/1;6/1;8 were significantly upregulated in roots, and GuPht1;5/1;6 were significantly upregulated in leaves. Low P treatment significantly increased the relative expression of GuPht1 in roots and leaves, and more members were upregulated in roots. GuPht1;5 was significantly upregulated in roots and leaves under drought, salt and hormone treatments. (4) GuPht1;1/1;2/1;4/1;5 genes were cloned and their lengths were 1 562 bp, 1 618 bp, 1 625 bp and 1 616 bp, encoding 522, 538, 540 and 537 amino acids, respectively. This study provided reference for further study on the function of Pht1 family and the response mechanism of G. uralensis to nutrient stress.