麦冬和青绿薹草对土壤苯并[a]芘的去除率及叶片生理响应特征
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北京林业大学建设世界一流学科和特色发展引导专项资金(2019XKJS0322);


Removal Rate of Benzo[a]pyrene from Soil and Characteristics of Leaf Physiological Response by Ophiopogon japonicus and Carex breviculmis
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    摘要:

    为了明确北京园林绿地地被植物麦冬和青绿薹草对土壤苯并[a]芘的净化能力,以普通园土为对照,设置5.5、15和30 mg/kg等3个土壤苯并[a]芘污染浓度,通过盆栽试验研究两种植物对苯并[a]芘的耐受性、去除效果及叶片中光合色素和氧化应激等生理响应特征。结果表明:(1)两种植物在30 mg/kg及以下的土壤苯并[a]芘浓度中均可生存且具有观赏价值,但青绿薹草株高及干重均受到显著抑制,而麦冬仅株高受到显著抑制。(2)两种植物均可以显著降低土壤苯并[a]芘浓度,且去除率随浓度升高呈先升高后降低的趋势;麦冬在5.5 mg/kg时去除率最高(76.9%),青绿薹草在15 mg/kg时去除率最高(79.6%)。(3)随土壤苯并[a]芘浓度升高,麦冬叶片各类光合色素含量均先降低后升高,而青绿薹草则先升高后降低,但仅在个别处理下变化显著。(4)各苯并[a]芘浓度处理下麦冬叶片中MDA含量均与对照组无显著差异,而各处理下青绿薹草均显著高于对照组。(5)各苯并[a]芘浓度处理下麦冬、青绿薹草叶片中过氧化氢和羟自由基含量基本上均高于对照组,其中麦冬在15和30 mg/kg浓度处理下过氧化氢含量以及15 mg/kg浓度处理下羟自由基含量增幅显著,青绿薹草在15 mg/kg浓度处理下过氧化氢含量以及各浓度处理下的羟自由基含量均显著高于对照组。(6)在各土壤苯并[a]芘浓度下,两种植物叶片POD和CAT活性均与对照组无显著差异,SOD活性仅在麦冬5.5 mg/kg浓度处理下和青绿薹草30 mg/kg浓度处理下显著高于对照组。研究发现,麦冬和青绿薹草均可耐受苯并[a]芘污染浓度低于30 mg/kg的土壤环境,同时有效去除土壤中的苯并[a]芘;麦冬的去除效果在土壤苯并[a]芘浓度较低时优于青绿薹草,而在高浓度时逊于青绿薹草;麦冬对苯并[a]芘土壤污染的较强耐受性与其在各污染浓度下叶片光合色素含量较稳定、膜质过氧化程度较低等密切相关。

    Abstract:

    In order to understand the purification ability of Ophiopogon japonicus and Carex breviculmis on soil benzo[a]pyrene in Beijings garden green space, taking ordinary garden soil as the control, we set up three soil benzo[a]pyrene contamination concentrations of 5.5 mg/kg, 15 mg/kg and 30 mg/kg to study the tolerance, removal effect and characteristics of physiological responses such as photosynthetic pigments and oxidative stress in leaves of two plants on benzo[a]pyrene through pot experiments. The results show that: (1) both plants can survive and have ornamental value at soil benzo[a]pyrene concentrations of 30 mg/kg and below, but the plant height and dry weight of C. breviculmis were significantly inhibited, while only the plant height of O. japonicus was significantly inhibited. (2) Both plants can reduce the concentration of soil benzo[a]pyrene significantly, and the removal rate showed a trend of first increasing and then decreasing with the increase of concentration. The removal rate of O. japonicus was the highest at 5.5 mg/kg (76.9%), and the removal rate of C. breviculmis was the highest at 15 mg/kg (79.6%). (3) With the increase of soil benzo[a]pyrene concentration, the contents of various photosynthetic pigments in the leaves of O. japonicus first decreased and then increased, while C. breviculmis first increased and then decreased, but the changes were significant only under individual treatments. (4) There was no significant difference in MDA content in leaves of O. japonicus under each concentration of benzo[a]pyrene compared with the control group, while C. breviculmis was significantly higher than the control group under each treatment. (5) The contents of hydrogen peroxide and hydroxyl radical in the leaves of O. japonicus and C. breviculmis under each benzo[a]pyrene concentration treatment were basically higher than those in the control group, and among them, the content of hydrogen peroxide under the treatment of 15 mg/kg and 30 mg/kg of O. japonicus and the content of hydroxyl radicals under the treatment of 15 mg/kg concentration increased significantly, and the content of hydrogen peroxide and the content of hydroxyl free radicals under the treatment of 15 mg/kg for C. breviculmis were significantly higher than those in the control group. (6) At each concentration of benzo[a]pyrene in soil, the activities of POD and CAT in leaves of the two plants were not significantly different from those in the control group, and the SOD activity was significantly higher than the control group only under the treatment of 5.5 mg/kg for O. japonicus and under the treatment for 30 mg/kg of C. breviculmis. The study found that both O. japonicus and C. breviculmis can tolerate the soil environment with benzo[a]pyrene pollution concentration lower than 30 mg/kg, and at the same time effectively remove benzo[a]pyrene in the soil; the removal effect of O. japonicus was better than C. breviculmis when the soil benzo[a]pyrene concentration was low, but inferior to C. breviculmis when the soil concentration was high; The strong tolerance of O. japonicus to benzo[a]pyrene soil pollution was closely related to the stable content of photosynthetic pigments in leaves and the lower degree of membrane peroxidation under various pollution concentrations.

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张灵巧,朱梦婷,彭 颖,等.麦冬和青绿薹草对土壤苯并[a]芘的去除率及叶片生理响应特征[J].西北植物学报,2022,42(7):1198-1207

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  • 在线发布日期: 2022-08-22
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