We analyzed 7 cultivars of Cymbidium hybrid to explore on components basis of flower coloration. The pigment type was determined by chromogenic reaction and UVvisible spectra, and pigment components and content differences between yellow ‘Yufeng’ and red ‘Fuyunhongxia’ were conducted by ulterperformance liquid chromatography tandem mass spectrometry (UPLCMS/MS). This will provide theoretical references for further revealing mechanism of color formation and breeding in Cymbidium hybrid. The results showed that: (1) 7 cultivars all contained flavonoids, but did not contain carotenoids. Except for ‘Yufeng’ and ‘Shuangyiyafeng’, the rest contained anthocyanins. (2) A total of 160 differential metabolites were identified between the two cultivars and they exhibited more significant enrichment in anthocyanin biosynthesis and flavone and flavonol biosynthesis. (3) In ‘Fuyunhongxia’, the main flavonoids metabolotes are cyandin derivatives and peonidin derivatives, while flavonoids metabolites in ‘Yufeng’ are quercetin derivatives. The results indicated that the diversion of anthocyanin and flavonol is a major reason for the color change in Cymbidium hybrid.