In order to study the plant type optimization and compensation effect of storage of non structural carbohydrate (NSC) in plants of potted tree peony, and lay a theoretical foundation for the management of potted seedling type of tree peony. Taking the 2-year-old tree peony cv. ‘Luoyanghong’ grafted seedlings as the test material, L9 (34) orthogonal test was set up to analyze the plant type development and NSC accumulation characteristics of ‘Luoyanghong’ plants with root pruning, rooting agent and Metarhizium anisopliae as treatment factors. The results showed that the height of tree peony decreased and the root system had obvious fibrous rooting under potted conditions. Compared with the control, the plant height of each treatment decreased, the number of branches increased, and the plant type was more compact for potted tree pony. Mild pruning could promote the compensatory growth of plants, while excessive pruning could significantly inhibit the growth of potted tree peony plants. Therefore, light pruning could promote the compensatory growth , while excessive pruning could significantly inhibit the growth of potted tree peony plants. Also, the rooting agent could significantly increase the biomass of roots and leaves and the accumulation of NSC of the whole plants, and could improve the sink and source in both directions to promote the compensatory growth of potted tree peony plants. However, Metarhizium anisopliae had the least effect on the NSC accumulation of the whole plants. Leaf area per plant, fresh sample quality of old roots and lignified new roots contribute greatly to the accumulation of NSC in potted tree peony, so promoting the development of roots and leaves might an important part of potted tree peony. The biomass increment of T1, T2, T3, T4, T6 combinations exceeded that of the control, and the compensation effect was significant enhanced for potted plants. Among them, T3 was the better combination (25% root pruning, with irrigating 750 mg?L-1 rooting agent and 10 million units?mL-1 Metarhizium anisopliae). Therefore, light root pruning combined with moderate rooting agent and Metarhizium anisopliae liquid irrigation could realize potted seedlings with height moderate dwarfing, increased branches, developed roots, more NSC accumulation, and compact and coordinated plant type for potted tree peony production.