The study was based on full-length transcriptome data from Nitraria tangutorum, and the seedlings of N. tangutorum were used as experimental materials, NtBES1 family members from the transcriptome were identified and bioinformatics analysis were carried out; in addition, quantitative Real-time PCR was applied to analysis the expression patterns of NtBES1s in leaves treated with different concentrations of PEG and NaCl, which lay the foundation for the in-depth study of function of NtBES1 family members and molecular mechanism of stress resistance in N. tangutorum. The results showed that: (1) a total of 7 NtBES1s with complete BES1_N conserved domains were identified, and they were named as NtBES1-1-NtBES1-7; (2) the amino acids number of NtBES1 protein ranged from 86 to 422 aa, the molecular weight ranged from 9.75 kD to 47.53 kD, the isoelectric point ranged from 5.29 to 10.22, the instability index ranged from 34.88 to 75.02, and the average hydrophilic coefficient was negative; (3) the prediction results of subcellular localization showed that NtBES1-6 was localized in the cytoplasm, and the remaining 6 members were localized in the nucleus; (4) the seven NtBES1s were divided into three groups by phylogenetic tree, NtBES1-2, NtBES1-3 and NtBES1-7 were a group, NtBES1-1, NtBES1-5 and NtBES1-6 were a group, and NtBES1-4 was a single group, and the structure, physicochemical properties and functional annotations of the members in same groups were similar; (5) the three genes with high expression levels screened in the pre-experiment showed different expression patterns under stress: NtBES1-2 and NtBES1-6 were upregulated only at 30% PEG treatment for 2 h; under NaCl treatment, NtBES1-2 and NtBES1-4 showed the highest expression at 200 mmol/L treatment for 2 h, after 450 mmol/L treatment for 12 h, the expression level of these three genes gradually began to up-regulate, and reached to their highest levels at 24 h. The research shows that there are differences in the structural characteristics of NtBES1s, and different members play different roles in drought and salt tolerance process.