Pre-mRNA from the same gene can produce different transcript isoforms after alternative splicing (AS), which changes the location, stability and post-translational modification function of the encoded protein in cells, enhancing the ability to growth & development and respond to environmental stress. Serine-arginine-rich proteins (SR proteins) is a kind of important splicing factor (SF) family that determines the efficiency and accuracy of AS. Based on a brief introduction of the concept and classification of SR proteins family, the authors systematically reviewed the structural characteristics, classification, subcellular localization and transcriptional regulation of SR-like (SR45/45a) subfamily in plant-specific SR proteins for the first time, summarized the AS regulatory mechanism of genes under biotic/abiotic stresses, and prospected several possible directions for the research of SR-like in the future.