蓖麻RcbZIP11基因克隆及表达特性研究
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1.内蒙古民族大学 农学院;2.通辽市农业技术推广中心

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S565.6

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国家自然科学基金(31860389; 32060492)和内蒙古自治区自然科学基金(2020LH03006; 2022MS03057)


Cloning and expression characteristics of RcbZIP11 gene in castor
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    摘要:

    bZIP(basic leucine zipper)作为植物转录因子家族中成员数量最多的种类之一,主要参与植物的生长发育、逆境胁迫响应和激素信号转导调控等。本研究基于蓖麻转录组数据,克隆到1条在低温胁迫下显著上调表达的bZIP基因,并将其命名为RcbZIP11(GenBank No. OQ506490)。RcbZIP11基因的编码区序列全长492 bp,其推导163个氨基酸,预测是1个亲水性蛋白质,具有保守的bZIP_plant_GBF1结构域。蛋白质的多序列对比显示,RcbZIP11蛋白质在进化过程中相对保守,进化树分析显示RcbZIP11与麻风树JcbZIP11蛋白质亲缘关系最近。亚细胞定位显示RcbZIP11蛋白质定位于细胞核。并成功克隆出RcbZIP11启动子序列,预测结果显示该区域拥有光响应元件、逆境响应类元件和激素诱导类元件。RcbZIP11基因在蓖麻不同组织(真叶、子叶、茎和根)中均有表达,在子叶中相对表达量最高;且该基因在逆境胁迫下(干旱、盐、低温和ABA)的根和真叶中其相对表达量均高于0 h,说明RcbZIP11基因积极地参与蓖麻的非生物胁迫响应。综上,本研究为进一步探究RcbZIP11基因在蓖麻逆境条件生长过程中的功能奠定了基础。

    Abstract:

    bZIP ( basic leucine zipper ), as one of the most abundant members of the plant transcription factor family, is mainly involved in plant growth and development, stress response, and hormone signal transduction regulation. In this study, based on the transcriptome data of castor, a bZIP gene that was significantly up-regulated under low-temperature stress was cloned and named RcbZIP11(GenBank No. OQ506490). The coding region sequence of the RcbZIP11 gene was 492 bp in length, which deduced 163 amino acids. It was predicted to be a hydrophilic protein with a conserved bZIP _ plant _ GBF1 domain. Multiple sequence alignment showed that RcbZIP11 protein was relatively conservative during evolution. Phylogenetic tree analysis showed that RcbZIP11 had the closest relationship with JcbZIP11 protein. Subcellular localization showed that the RcbZIP11 protein was localized in the nucleus. The promoter sequence of the RcbZIP11 was successfully cloned. The prediction results showed that the region had light response elements, stress response elements, and hormone induction elements. RcbZIP11 gene was expressed in different tissues ( true leaves, cotyledons, stems, and roots ) of castor, and the relative expression level was the highest in cotyledons. The relative expression levels of RcbZIP11 in roots and leaves under stress ( drought, salt, low temperature, and ABA ) were higher than 0 h, indicating that the RcbZIP11 gene was actively involved in the abiotic stress response of castor. In summary, this study lays a foundation for further exploring the function of the RcbZIP11 gene in the growth process of castor under stress conditions.

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李艳肖,朱贵爽,张宏宇,等.蓖麻RcbZIP11基因克隆及表达特性研究[J].西北植物学报,2023,43(9):1478-1487

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  • 收稿日期:2023-04-16
  • 最后修改日期:2023-07-09
  • 录用日期:2023-08-03
  • 在线发布日期: 2023-09-26
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