Abstract: Invertase (INV) plays an important role in the plant growth and development and resistance to the stress. In this study, 19 INV family members were identified in the grape genome database, and the structures of the genes and the physical - chemical properties of the coding protein were analyzed by bioinformatics. The expression characteristics of the genes under different hormone and abiotc stress conditions were analyzed by fluorescence quantitative PCR(qRT-PCR) technology, which could provide a theoretical basis for further exploring the involvement of VvINV gene family in grape stress response. The results are as followings: (1) The gene family encoded proteins with amino acid lengths between 150~766 aa and the theoretical isoelectric point ranged from 4.43 to 9.1. (2) Collinearity analysis showed that VvCINVs had a higher replication frequency with other 5 species. (3) The conserved motifs analysis showed that VvCwINVs contained all conserved motifs, and Glyco_32 and Glyco_hydro_100 were the main domains of VvINV genes. (4) Tissue-specific expression analysis showed that the most genes were expressed during the growth and development of grape. (5) The results of qRT-PCR showed that VvINV family genes were up-regulated in response to hormone treatment and abiotic stresses in leaves; VvCINV1 was highly expressed under 50 mg/L GA3 and 10% PEG treatments; VvCINV4 was also significantly expressed under salt, ABA and low temperature treatments. These results indicated that VvINVs can regulate hormone and abiotic stress response during grape growth and development. Keywords:grape;