【Objective】 Flavonols play an important role in the growth and development of plant, and the resistance to stress of plant. In order to explore relevant information of flavonols synthase gene of Menyuan rapeseed in Qinghai province, and provide a reference for further analysis of BpFLS gene function. 【Methods】 The authors utilized the transcriptome to analyze the pollen of Menyuan rapeseed in Qinghai province and Zhengzhou rapeseed in Henan province as materials. At the same time, the differentially expressed flavonol synthase genes were selected for bioinformatics analysis and RACE-PCR cloning. 【Results】 The results showed that: (1) 6 收稿日期:2023-06-20 修改稿收到日期:2023-10-24 基金项目:中央高校科研经费项目（lzujbky-2022-kb05）；河南省科技厅自然科学类普通科技攻关项目（212102110050） 作者简介:刘晓宁(1985—)，女，博士，副教授，主要从事植物分子生物学研究。E-mail：Xiaoningliu2016@126.com differentially expressed flavonol synthase genes in rape pollen were screened by transcriptome analysis, and BpFLS1-1 gene was the most differentially expressed and up-regulated in the pollen of Menyuan rapeseed. (2) The amino acid sequence encoded by BpFLS1-1 gene has both flavonol synthase activity and flavanone-3-hydroxylase activity. (3) BpFLS1-1 gene might contain a new coding region sequence with a length of 1 170 bp. 【Conclusion】 It is speculated that BpFLS1-1 gene may play an important role in the accumulation of flavonoids in pollen and stress resistance in Menyuan rapeseed.