【Objective】NAC transcription factor family is a class of transcription factors that are widely studied in plants. It plays an important role in regulating plant growth and development and responding to abiotic stress. Maize is one of the three major food crops, which will face various adverse stresses in its growth process. Salt damage is considered to be one of the main environmental factors limiting crop growth and production. Therefore, it is of great significance to identify maize salt resistance genes and analyze their salt resistance mechanism for breeding maize stress resistant varieties. 【Method】This study cloned the maize transcription factor ZmNAC59 and analyzed its conserved domain and phylogenetic relationship using bioinformatics methods. The expression pattern of the gene in leaves under NaCl and MeJA treatment was analyzed using the Quantitative Real-time PCR method. The stable transgenic system was used to heterologously express the gene into Arabidopsis for phenotype observation. At the same time, virus-induced silencing technology was used to silence the gene in maize, followed by salt treatment phenotype observation and enzyme activity detection. 【Result 】The qRT-PCR results showed that ZmNAC59 could be up-regulated by NaCl and MeJA. After virus-induced silencing of ZmNAC59, the silenced strains were more sensitive to salt stress and accumulated more ROS. After overexpression of ZmNAC59 in Arabidopsis thaliana, the overexpression lines had higher survival rate, less accumulation of reactive oxygen species and lower Na+/K+ ratio under salt stress, indicating that ZmNAC59, as a positive regulator of salt stress, can improve plant salt resistance by regulating ion flow. qRT-PCR results showed that Na+, K+ transport related genes were significantly up-regulated in Arabidopsis overexpression lines; After transient overexpression of ZmANC59 in maize protoplasts, the expression of ZmSOS1, ZmNHX1 and ZmNHX7 were significantly up-regulated after salt treatment. The Dual-Luc experiment showed that ZmSOS1 was the target gene of ZmNAC59. 【Conclusion】 This study found that ZmNAC59 could promote ion transport under salt stress by activating the expression of ZmSOS1, which provided a scientific theoretical basis for the screening of maize stress resistance gene resources and cultivar cultivation.